Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 11  |  Issue : 1  |  Page : 20--24

The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide-propolis combination in human pulp fibroblast


Ira Widjiastuti, Mieke Kusuma Dewi, Edhi Arief Prasetyo, Nirawati Pribadi, Mochamad Moedjiono 
 Departement of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Correspondence Address:
Dr. Ira Widjiastuti
Jl. Mayjen Prof. Dr. Moestopo 47, Surabaya
Indonesia

Calcium hydroxide (Ca(OH)2) is the gold standard material used for pulp-capping but still has a high failure rate. Thus, an alternative material is needed, one of which is propolis. The combination of Ca(OH)2propolis is expected to have better quality and to be biocompatible. The aim of this study is to investigate the viability of human pulp fibroblast after the administration of Ca(OH)2, propolis, and its combination. Human pulp fibroblast culture derived from premolar teeth of 16-year-old patients, were divided into seven groups: Group 1 (10 μg Ca(OH)2); Group 2 (10 μg propolis); Group 3 (15 μg propolis); Group 4 (20 μg propolis); Group 5 (Ca(OH)2-propolis 1:1); Group 6 (Ca(OH)2-propolis 1:1.5); and Group 7 (calcium hydroxide-propolis 1:2). They were placed in a 96 wells plate and put into incubator for 24 h. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test was conducted to calculate the viability of human pulp fibroblasts. The data were analyzed statistically using Kolmogorov–Smirnov, Levene's test, one-way analysis of variance, and Tukey-honestly significant difference (P < 0.05). The number of living human pulp fibroblast after the administration of Ca(OH)2and propolis combination is greater than the application of Ca(OH)2or propolis with significant different between groups (P < 0.05). The viability of human pulp fibroblasts after the administration of Ca(OH)2-propolis combination is greater than that of the application of Ca(OH)2and propolis alone.


How to cite this article:
Widjiastuti I, Dewi MK, Prasetyo EA, Pribadi N, Moedjiono M. The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide-propolis combination in human pulp fibroblast.J Adv Pharm Technol Res 2020;11:20-24


How to cite this URL:
Widjiastuti I, Dewi MK, Prasetyo EA, Pribadi N, Moedjiono M. The cytotoxicity test of calcium hydroxide, propolis, and calcium hydroxide-propolis combination in human pulp fibroblast. J Adv Pharm Technol Res [serial online] 2020 [cited 2020 Oct 28 ];11:20-24
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2020;volume=11;issue=1;spage=20;epage=24;aulast=Widjiastuti;type=0