Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 8  |  Issue : 4  |  Page : 143--149

Acacia catechu ethanolic bark extract induces apoptosis in human oral squamous carcinoma cells


Thangavelu Lakshmi1, Devaraj Ezhilarasan2, Rajagopal Vijayaragavan3, Sukhwinder Kaur Bhullar4, Ramasamy Rajendran5 
1 Department of Pharmacology, Saveetha Dental College and Hospital, Saveetha University, Chennai, Tamil Nadu, India
2 Department of Pharmacology, Saveetha Dental College and Hospital, Saveetha University; Department of Pharmacology, Biomedical Research Unit and Animal Research Centre, Saveetha Dental College and Hospital, Saveetha University, Chennai, Tamil Nadu, India
3 Department of Research, Saveetha University, Chennai, Tamil Nadu, India
4 Department of Mechanical Engineering, Bursa Technical University, Bursa, Turkey
5 Green Chem Herbal Extracts and Formulations, Bengaluru, Karnataka, India

Correspondence Address:
Devaraj Ezhilarasan
Department of Pharmacology, Biomedical Research Unit and Lab Animal Centre, Saveetha Dental College and Hospitals, Saveetha University, Chennai - 600 077, Tamil Nadu
India

Oral cancer is in approximately 30% of all cancers in India. This study was conducted to evaluate the cytotoxic activity of ethanolic extract of Acacia catechu bark (ACB) against human squamous cell carcinoma cell line-25 (SCC-25). Cytotoxic effect of ACB extract was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide assay. A. catechu extract was treated SCC-25 cells with 25 and 50 μg/mL for 24 h. Apoptosis markers such as caspases-8 and 9, bcl-2, bax, and cytochrome c (Cyt-c) were done by RT-PCR. Morphological changes of ACB treated cells were evaluated using acridine orange/ethidium bromide (AO/EB) dual staining. Nuclear morphology and DNA fragmentation were evaluated using propidium iodide (PI) staining. Further, cell cycle analysis was performed using flow cytometry. A. catechu treatment caused cytotoxicity in SCC-25 cells with an IC50 of 52.09 μg/mL. Apoptotic marker gene expressions were significantly increased on ACB treatment. Staining with AO/EB and PI shows membrane blebbing and nuclear membrane distortion, respectively, and it confirms the apoptosis induction in SCC-25 cells. These results suggest that ACB extract can be used as a modulating agent in oral squamous cell carcinoma.


How to cite this article:
Lakshmi T, Ezhilarasan D, Vijayaragavan R, Bhullar SK, Rajendran R. Acacia catechu ethanolic bark extract induces apoptosis in human oral squamous carcinoma cells.J Adv Pharm Technol Res 2017;8:143-149


How to cite this URL:
Lakshmi T, Ezhilarasan D, Vijayaragavan R, Bhullar SK, Rajendran R. Acacia catechu ethanolic bark extract induces apoptosis in human oral squamous carcinoma cells. J Adv Pharm Technol Res [serial online] 2017 [cited 2021 Oct 23 ];8:143-149
Available from: https://www.japtr.org/article.asp?issn=2231-4040;year=2017;volume=8;issue=4;spage=143;epage=149;aulast=Lakshmi;type=0