Home  |  About JAPTR |  Editorial board  |  Search |  Ahead of print  |  Current issue  |  Archives |  Submit article  |  Instructions  |  Subscribe  |  Advertise  |  Contacts  |Login 
Users Online: 262   Home Print this page Email this page Small font sizeDefault font sizeIncrease font size

 Table of Contents  
Year : 2011  |  Volume : 2  |  Issue : 1  |  Page : 62-67  

Antibacterial activities and phytochemical analysis of Cassia fistula (Linn.) leaf

1 Department of Biotechnology and Zoology, North Orissa University, Baripada, Orissa, India
2 Department of Zoology, North Orissa University, Baripada, Orissa, India
3 Department of Biotechnology, North Orissa University, Baripada, Orissa, India

Date of Web Publication21-Apr-2011

Correspondence Address:
Sujogya K Panda
Department of Biotechnology, North Orissa University, Baripada - 757 003, Orissa
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2231-4040.79814

Rights and Permissions

Cassia fistula Linn. which belongs to family Leguminosae is a medium-sized tree and its different parts are used in ayurvedic medicine as well as home remedies for common ailments. Sequential extraction was carried out using solvents viz. petroleum ether, chloroform, ethanol, methanol and water from leaf of the plant were investigated for preliminary phytochemical and antibacterial property. Results of the study showed that all the extracts had good inhibitory activity against Gram-positive test organism. Although all five extracts showed promising antibacterial activity against test bacterial species, yet maximum activity was observed in ethanol extract. The minimum inhibitory concentration ranged in between 94 to 1 500 μg/ml. Evaluation of phytochemicals such as alkaloids, flavonoids, carbohydrates, glycosides, protein and amino acids, saponins, and triterpenoids revealed the presence of most of constituents in polar extracts (ethanol, methanol, and aqueous) compared with nonpolar extracts (petroleum ether and chloroform). Furthermore, the ethanol extract was subjected to TLC bioautography and time-kill study against Staphylococcus epidermidis. All the findings exhibit that the leaf extracts have broad-spectrum activity and suggest its possible use in treatment of infectious diseases.

Keywords: Cassia fistula , human pathogenic bacteria, minimum inhibitory concentration, Similipal Biosphere Reserve, TLC bioautography

How to cite this article:
Panda SK, Padhi L P, Mohanty G. Antibacterial activities and phytochemical analysis of Cassia fistula (Linn.) leaf. J Adv Pharm Technol Res 2011;2:62-7

How to cite this URL:
Panda SK, Padhi L P, Mohanty G. Antibacterial activities and phytochemical analysis of Cassia fistula (Linn.) leaf. J Adv Pharm Technol Res [serial online] 2011 [cited 2023 Mar 22];2:62-7. Available from: https://www.japtr.org/text.asp?2011/2/1/62/79814

   Introduction Top

Cassia fistula Linn. (Leguminosae) is a very common plant and is widely known for its medicinal properties. In the Indian literature, this plant has been described to be useful against skin diseases, liver troubles, tuberculous glands and its use in the treatment of rheumatism, hematemesis, pruritus, leucoderma, and diabetes. [1],[2] Besides, it has been found to exhibit anti-inflammatory and hypoglycemic activity and widely used as a mild laxative suitable for children and pregnant women. [3] Several reports are present on hepatoprotective, [4] antifertility, [5] and antioxidant properties of C. fistula. [6],[7] Some studies have also been done on antimicrobial activity of C. fistula flower and seed along with some other Indian medicinal plants. [8],[9],[10],[11],[12],[13],[14],[15],[16] These studies give diminutive information on the antimicrobial property of the leaves of this plant.

Reports of the preliminary screening of this plant collected from Similipal Biosphere Reserve (SBR) [17] recorded of its antimicrobial activity against certain bacterial strains. The plant has diverse ethnomedicinal uses by the tribals of SBR. The bark paste is applied externally on the bite area for 2 to 3 times in a day at regular interval for 3 days. Half teaspoon juice extract is taken orally thrice daily to cure jaundice. The leaf paste along with neem is applied externally over all types of skin infections. Several reports are available on antimicrobial activities of C. fistula from bark, seed, flower, and fruits, but that on leaves are scanty.

In the present study, an attempt has been made to investigate the antibacterial activity of different solvent extracts of leaf of C. fistula obtained by sequential extraction method, against Gram-positive and -negative bacteria. In addition to this, TLC bioautography study and presence of phytochemical constituents in the respective extracts were also carried out.

   Materials and Methods Top

Plant Material

Leaves of C. fistula were collected in the month of April, 2007 from SBR, Mayurbhanj, Orissa and their identity was confirmed and the voucher specimen (NOU 25) was deposited in the Department of Botany, North Orissa University. The shed dried healthy leaves were powdered separately using mechanical grinder and then were passed through sieve in order to maintain uniform powder size.

Preparation of Extracts

Sequential extraction was carried out with the same powder using solvents of increasing polarity. About 250 g of dry leaf powder were sequentially extracted using petroleum ether, chloroform, ethanol, methanol, and aqueous solution in Soxhlet apparatus. After about forty siphons of each solvent extraction step, the materials were concentrated by evaporation. The concentrated extracts were freeze dried to remove the solvent at -2oC till further use. The yield of each extract was calculated and stored for further use.

Bacteria and Growth Media

 Escherichia More Details coli (MTCC 1098), Escherichia coli O157:H7 (RMRC, Bhubaneswar, India),  Salmonella More Details typhimurium (MTCC 3216), Shigella sonnei (NICED, Kolkata, India), Bacillus subtilis (MTCC 7164), Bacillus licheniformis (MTCC 7425), Staphylococcus aureus (MTCC 1144), and Staphylococcus epidermidis (MTCC 3615) were used as test microorganisms. All these bacterial cultures were grown at 37oC and maintained at 4oC on nutrient agar slants (Hi-media Pvt. Ltd., Mumbai, India).

Agar Cup Method

The agar cup method (ACM) was used to study the antibacterial activity of the extracts. [18] Briefly, cultures of the bacteria from culture plates were scooped using a wire loop and separately mixed with normal saline and agitated with vortex mixer. A loop full was withdrawn and uniformly distributed on the surface of the agar plate by streaking using a sterile swab. Wells of approximately 6 mm in diameter and 2.5-mm deep were made on the surface of the solid medium using a sterile borer. The plates were turned upside down and the wells labeled with a marker. The extracts were reconstituted by dissolving in dimethyl sulfoxide (DMSO). Each well was filled with test sample. Sterile DMSO was used as negative control, while gentamicin and ciprofloxacin were used as positive control. The plates were incubated at 37ºC for 24 hours. After 24 hours, the plates were removed and zones of inhibition measured with Himedia antibiotic scale and the results were tabulated. Extracts with zones of inhibition greater or equal to 8-mm diameter were regarded as positive. The mean±SD of the inhibition zone was taken for evaluating the antibacterial activity of the extracts.

Determination of Minimum Inhibitory Concentration

Although the results of the ACM cannot always be compared with the minimum inhibitory concentration (M ic0 ) data, extracts which showed positive result were further evaluated for the determination of MIC. A broth microdilution technique was adopted using 96-well microtiter plates and tetrazolium salt, 2, 3, 5-triphenyltetrazolium chloride (TTC) was carried out to determine the MIC following the methods with modification, as described by Eloff. [19] In the plate, A 1 to H 1 were blank with MH broth only. A 3 to H 3 were having the stock solution of the test extract(s) and A 4 to H 4 till A 9 to H 9 were the wells in which the test extracts were serially diluted using MH broth. Wells A 12 to D 12 were controls having 20 μl of DMSO and E 12 to H 12 served as control over control. All wells were dispensed with 100 μl of MH broth. 20 μl of the herbal extract was transferred from stock test solution to the first well, that is, from A 4 to H 4 containing 100 μl of MH broth. 20 μl of the MH broth containing herbal extract was then transferred to the next well to create serial dilutions. 100 μl of the 0.5 McFarland adjusted activated culture in MH broth was then added to all the wells except the blank. 5 μl of 0.5% TTC was further added to all the dilutions, blank, control, and control over control. The final volume of all the wells was 205 μl. The microplate was sealed and incubated at 37ºC at 130 rpm. 10 μl of the broth from each culture tube exhibiting MIC and control tubes were taken aseptically and were plated on one-day old Muller-Hinton (MH) agar plate as a point inoculum and allowed to dry for 10 minutes under the laminar air hood. The microplate was sealed and incubated at 37 o C at 130 rpm and observed for growth of the microorganism. The lowest recorded MIC was further subjected to time kill kinetics using ethanol, methanol, and aqueous extracts of C. fistula. 20 μl of overnight broth culture of S. epidermidis was added to 180 μl of NB containing each extract. The microtiter plate was incubated at 37ºC. The number of viable cells was determined after 0, 1, 2, 4, 8, 12, 16, 24, and 48 hours of incubation. A control culture without crude extract was incubated and assayed under the same condition. [20]

TLC Bioautography

TLC bioautography assay was performed by agar overlay bioautography technique. Plant extract samples (5 μl) were applied 2.5 cm from the base of the silica plate. After drying, the plates were developed using solvent chloroform : methanol (8.2 : 1.8) and chloroform : hexane (5.4 : 6.6). Developed TLC plates were carefully dried for complete removal of solvents. Aliquot of 50 ml of molten MH agar was prepared by adding 500 μl of bacterial inoculum (5 × 10 5 CFU). Now, the inoculum containing agar was overlaid on dried TLC plate under aseptic condition in laminar airflow. The plates were incubated at 37oC and examined for the zone of inhibition.

   Results and Discussion Top

The amounts of crude material extracted per gram of powdered C. fistula leaf were 15.2, 24.5, 43.8, 68.2, and 158.6 mg, respectively, with chloroform, petroleum ether, methanol, distilled water, and ethanol solvents. Evaluation of phytochemicals such as alkaloids, flavonoids, carbohydrates, glycosides, protein and amino acids, saponins, and triterpenoids revealed the presence of most of the constituent in polar extracts such as ethanol, methanol, and aqueous extracts compared with nonpolar extracts (petroleum ether and chloroform). However, flavonoids, proteins and amino acids, tannins, and phenols were found to be universally occurring in all the extracts [Table 1].
Table 1: Phytochemical screening of C. fistula leaf

Click here to view

[Table 2] lists the plant extracts and their zone of inhibition against the test bacterium. Chloroform extract did not show any zone of inhibition against Gram-negatives, whereas Gram-positive bacteria were completely inhibited by all test extracts. Several techniques have been reported for the testing of antimicrobial activity of natural products including plant extracts at different time and stipulation. However, ACM is always employed for preliminary testing of antimicrobial activities of crude extracts, while advanced method like MIC is mostly used as secondary confirmation method. In ACM, all test bacteria showed zone of inhibition with petroleum ether, ethanol, methanol, and aqueous extracts. Among Gram-positives, S. epidermidis, B. licheniformis, and B. subtilis were absolutely inhibited by all extracts. Ethanol and methanol extracts of the leaf were most active inhibiting agent against both Gram-positive and -negative bacteria. On the other hand, petroleum ether extract had better antibacterial activity against most of the Gram-negative bacteria. Among Gram-negatives, the highest zone of inhibition was recorded for ethanol extract against E. coli O157:H7. From ACM, result was obtained that there was no significant difference among the test bacteria with respect to plant extracts, while there were marked differences between the activities of the plant extract and pure antibacterial drug (ciprofloxacin) [Figure 1]. Such significant differences are normally present when crude (unpurified) plant extracts are compared with pure drugs that are already in clinical use. [21] Also, the ACM is not always dependable for accurate assessment and comparison. This is because of the high degree of interference inherent in this method that arises from drug diffusion problems. [22]
Table 2: Antibacterial screening of different extracts of C. fistula leaf

Click here to view
Figure 1: Time kill kinetics against S. epidermidis

Click here to view

The MIC results showed that all the extracts were able to prevent the growth of all test organisms with selective activities. The growth of inhibition of test bacteria range from 94 to 1 500 μg/ml (w/v), with the lowest MIC value against S. epidermidis [Table 3]. The results of MBC showed that in a concentration of 1 500 μg/ml (w/v), most of the organisms from both Gram-positive and-negative bacteria were killed. These results clearly indicated that S. epidermidis was the most sensitive organism, which is inhibited with MIC values lower than 100 μg/ml recorded in 50% of the test cases. MIC values lower than 200 μg/ml were also obtained with other extracts viz. ethanol, methanol, and aqueous against B. subtilis, B. licheniformis, and S. epidermidis. This activity could be considered very promising for two reasons, first, the test bacteria were resistant to the first line antibiotics and second, when compared with the reference drugs' (standard antibiotics) MICs (10 μg/ml). However, all lowest MICs exhibited by extracts with MBC value eight times of MIC in corresponding microorganisms highlighting their interesting antimicrobial potency. From these results, it can be observed that most of the tested samples exert a killing effect on the test organisms, as MBC and MFC values were recorded. However, when analyzing carefully the MIC and MMC results, it can be noted that MMC/MIC ratios lower than 4 was obtained with most of the studied samples, suggesting that killing effects could be expected. [23] The TLC results showed a total of 7 spots with R f values 0.09, 0.43, 0.40, 0.375, 0.55, 0.64, and 0.72. Ethanol, methanol, and aqueous extracts had three similar R f values (0.09, 0.43, 0.40) than other extracts, which was possible responsible for better antibacterial activity. Ethanol extract showed 5 distinct fractions with R f value 0.72, 0.55, 0.43, 0.37, and 0.09. The same TLC plate of ethanol extract was subjected against S. epidermidis to study bioautography and result showed that the activity was limited to the fraction with R f 0.72, which was evident as a bluish pink under exposure to UV light on the reference TLC plate with zone of inhibition 24 mm [Figure 2].
Table 3: MIC and MBC of different extracts of C. fistula leaf

Click here to view
Figure 2: TLC Bioautography with S. epidermidis

Click here to view

Extracts of ethanol, methanol, and aqueous were further tested for antimicrobial activity with time kill curve experiment against selective organisms. Results show that ethanol, methanol, and aqueous extracts exhibit lytic activity against selective strain. This suggested membrane disruption as the likely mechanism of action. In case of S. epidermidis, ethanol, methanol, and aqueous extract were bactericidal in nature, exhibiting 50% survival after 4 hours of incubation. After 8 hours of incubation, ethanol and methanol exhibited a prominent kill with 1.9 and 2.1% survival, respectively. This was followed by aqueous extract which exhibit 5.26% survival after 8 hours incubation. This concludes that ethanol is a potent bacteriostatic agent as compared with that of methanol and aqueous.

Kumar et al.[9] reported antimicrobial activity of fruit of C. fistula by agar dilution streak method at a concentration of 500 μg/ml. Only E. coli was moderately inhibited, whereas no inhibition was found in case of B. subtilis and S. epidermidis. However, in our study, B. subtilis was completely inhibited at concentration of 375 μg/ml by ethanol, methanol, and aqueous extract, while S. epidermidis was inhibited at concentration of 187.5 μg/ml by the same extract. Valsaraj et al.[15] studied the antibacterial activity of C. fistula seeds by broth dilution method and antifungal activity by ACM. According to their observation at 12.5 mg/ml concentration, E. coli and B. subtilis were inhibited while S. aureus was inhibited at a concentration 6.25 mg/ml. Perumal Samy et al.[12] reported moderate antibacterial activity of C. fistula against a wide spectrum of bacteria such as E. coli, Bacillus mycoides, B. subtilis, Mycobacterium smegmatis, Klebsiella aerogenes, Pseudomonas aerogenes, and Proteus vulgaris. A polar compound including 5-nonatetra contanone, 2-hentriacontanone, triacontane, 16-hentriacontanol, and sitosterol along with oil showing antibacterial activity had also been isolated in C. fistula pods. [10] Recently, Vimalraj et al.[16] tested the antibacterial activity of aqueous and alcoholic extract of stem bark of C. fistula with disc diffusion and MIC methods. Alcoholic extracts recorded greater inhibition against S. aureus compared with aqueous extract. Zones of inhibition of alcoholic and aqueous extracts were in the range of 7.0 to 12.0 mm and 7.0 to 11.6 mm, respectively. MIC values of the alcoholic extracts against S. aureus were in the range of 0.78 to 6.25 mg/ml . Our finding was almost coinciding with this study.

Though reports (Kumar et al., Perumal Samy et al., and Valsaraj et al.) [9],[12],[15] are available on antimicrobial activities of seeds, pods, bark of C. fistula, no work has been conducted on potentiality of leaves of this plant. Our report is of the first for antibacterial activity on leaf extracts of C. fistula. S. epidermidis is a part of normal skin flora, and is often attached to the upper layer of the skin (epidermis) or mucosa, without causing any symptom. When the skin is injured (wounds, burns, intravenous drug addicts, etc), S. epidermidis may enter into deeper layers of the skin or even the blood and cause an infection. S. epidermidis is the most common causative agent of post-cataract surgery endophthalmitis. [24] Since this bacterium is completely inhibited by leaf extracts, the plant can be useful for the treatment of S. epidermidis.

The presence of antibacterial substances in the higher plants is well established. Plants are the source of inspiration for novel drug compounds as plant-derived medicines have made significant contribution toward human health. Isolation of bioactive compounds from plant material largely depends on the type of solvent used in the extraction procedure. The traditional healers use primarily water as the solvent. This study concludes that the plant extraction by ethanol provided more consistent antimicrobial activity compared with water extract.

   Acknowledgments Top

We wish to express our profound gratitude to Dr. A. K. Biswal (North Orissa University) for identification of the plant. Thanks are also due to Dr. A. K. Bastia and Prof S. K. Dutta for providing necessary facilities to carry out this work and Dr. G. Sahoo for his cooperation and critical suggestion on the preparation of the manuscript.

   References Top

1.Alam MM, Siddiqui MB, Hussian W. Treatment of diabetes through herbal drugs in rural India. Fitoterapia 1990;61:240-2.  Back to cited text no. 1
2.Asolkar LV, Kakkar KK, Chakre OJ. Second supplement to glossary of Indian medicinal plant with active principles. New Delhi: Publication and Information Directorate, CSIR; 1992. p. 414.  Back to cited text no. 2
3.Kumar VP, Chauhan NS, Padhi H, Rajani M. Search for antibacterial and antifungal agents from selected Indian medicinal plants. J Ethnopharmacol 2006;67:241-45.  Back to cited text no. 3
4.Bhakta T, Mukherjee PK, Mukherjee K, Banerjee S, Mandal SC, Maity TK, et al. Evaluation of hepatoprotective activity of Cassia fistula leaf extract. J Ethnopharmacol 1999;66:277-82.  Back to cited text no. 4
5.Yadav R, Jain GC. Antifertility effect of aqueous extract of seeds of Cassia fistula in female rats. Adv Contraception 1999;15:293-301.  Back to cited text no. 5
6.J Munasinghe TC, Seneviratne CK, Thabrew MI, Abeysekera AM. Antiradical and anti-lipoperoxidative effects of some plant extracts used by Sri Lankan traditional medical practitioners for cardioprotection. Phytother Res 2001;15:519-23.  Back to cited text no. 6
7.Siddhuraju P, Mohan PS, Becker K. Studies on the antioxidant activity of Indian laburnum (Cassia fistula L.): A preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. J Agric Food Chem 2002;79:61-7.  Back to cited text no. 7
8.Duraipandiyan V, Ignacimuthu S. Antibacterial and antifungal activity of Cassia fistula L.: An ethnomedicinal plant. J Ethnopharmacol 2007;112:590-4.  Back to cited text no. 8
9.Kumar A, Pande CS. Kaul RK. Chemical examination of Cassia fistula flowers. Indian J Chem 1966;4:460  Back to cited text no. 9
10.Misra TR, Singh RS, Pandey HS, Pandev RP. Chemical constituents of hexane fraction of Cassia fistula pods. Fitoterapia 1996;57:173-4.  Back to cited text no. 10
11.Misra TR, Singh RS, Pandey HS, Singh BK. A new diterpene from Cassia fistula pods. Fitoterapia 1997;58:375-7.  Back to cited text no. 11
12.Perumal Samy R, Ignacimuthu S, Sen A. Screening of 34 Indian medicinal plants for antibacterial properties. J Ethnopharmacol 1998;62:173-82.  Back to cited text no. 12
13.Phongpaichit S, Pujenjob N, Rukachaisirkul V, Ongsakul M. Antifungal activity from leaf extracts of Cassia alata L., Cassia fistula L. and Cassia tora L. Songklanakarin J Sci Technol 2004;26:741-8.  Back to cited text no. 13
14.Sangetha SN, Zuraini Z, Sasidharan S, Suryani S. Antimicrobial activities of Cassia surattensis and Cassia fistula. J Mol Biol Biotechnol 2008;1:1-4.   Back to cited text no. 14
15.Valsaraj R, Pushpangadan P, Smitt UW, Adsersen A, Nyman U. Antimicrobial screening of selected medicinal plants from India. J Ethnopharmacol 1997;58:75-83.  Back to cited text no. 15
16.Vimalraj TR, Saravanakumar S, Vadivel S, Ramesh S, Thejomoorthy P. Antibcaterial effects of Cassia fistula extracts on pathogenic bacteria of veterinary importance. Tamilnadu J Veter Anim Sci 2009;5:109-1.  Back to cited text no. 16
17.Thatoi HN, Panda SK, Rath SK, Dutta SK. Antimicrobial activity and ethnomedicinal uses of some medicinal plants from Similipal Biosphere Reserve, Orissa. Asian J Plant Sci 2008;7:260-7.  Back to cited text no. 17
18.Barry AL. Procedure for testing antimicrobial Agents in Agar media. In: Antibiotics in Laboratory Medicine (Lorin V, ed). Baltimore: Williams Wilkins Co; 1980. p. 1-23.  Back to cited text no. 18
19.Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of the plant extracts for bacteria. Planta Med 1998;64:711-3.  Back to cited text no. 19
20.Panda SK, Dutta SK, Bastia AK. Antibacterial activity of Croton roxburghii balak. against the enteric pathogens. J Adv Pharm Tech Res 2010;1:419-22.  Back to cited text no. 20
  Medknow Journal  
21.Yoder S. Biomedical and ethnomedical practices in rural Zaire: contrasts and complements. Soc Sci Med 1982;16:1851-7.  Back to cited text no. 21
22.Dickert H, Machka K, Braveny I. The uses and limitations of disc diffusion in the antibiotic sensitivity testing of bacteria. Infection 1981;9:18-4.  Back to cited text no. 22
23.Carbonnelle B, Denis F, Marmonier A, Pinon G, Vague R. Techniques usuelles, Bactériologie médicale. SIMED, South Africa, 1987. p. 232.  Back to cited text no. 23
24.Han DP, Wisniewski SR, Wilson LA, Barza M, Vine AK, Doft BH, et al. Spectrum and susceptibilities of microbiologic isolates in the Endophthalmitis Vitrectomy study. Am J Ophthalmol 1996;122:1-17.  Back to cited text no. 24


  [Figure 1], [Figure 2]

  [Table 1], [Table 2], [Table 3]

This article has been cited by
1 Influence of Cassia fistula leaf powder on in vitro ruminal fermentation, gas production and degradability of diets for ruminants
R. M. Akinbode, A. A. Osinuga, S. M. Oladele, K. O. Adebayo, O. A. Isah
The Journal of Agricultural Science. 2023; : 1
[Pubmed] | [DOI]
2 Methanolic fraction of Cassia fistula L. bark exhibits potential to combat oxidative stress and possess antiproliferative activity
Rasdeep Kour, Neha Sharma, Sheikh Showkat, Sunil Sharma, Kommu Nagaiah, Subodh Kumar, Satwinderjeet Kaur
Journal of Toxicology and Environmental Health, Part A. 2023; 86(9): 296
[Pubmed] | [DOI]
3 Chemical profiling and biological activity of Cassia abbreviata Oliv.
Nawaal Benazir Bint Raman Ibrahim, Daneshwar Puchooa, Joyce Govinden-Soulange, Sunita Facknath
South African Journal of Botany. 2022; 146: 325
[Pubmed] | [DOI]
4 LC-QTOF-MS Characterization, Antioxidant Activity, and In Vitro Toxicity of Medicinal Plants from the Tri-Than-Thip Remedy
Palika Wetchakul, Piriya Chonsut, Chuchard Punsawad, Sineenart Sanpinit, Giuseppe Malfa
Evidence-Based Complementary and Alternative Medicine. 2022; 2022: 1
[Pubmed] | [DOI]
5 Molecular and metabolic traits of some Egyptian species of Cassia L. and Senna Mill (Fabaceae-Caesalpinioideae)
Marwa M. Eldemerdash, Ashraf S. A. El-Sayed, Hussein A. Hussein, Samir S. Teleb, Rania S. Shehata
BMC Plant Biology. 2022; 22(1)
[Pubmed] | [DOI]
6 Antibacterial and Antibiofilm Activity of Selected Medicinal Plant Leaf Extracts Against Pathogens Implicated in Poultry Diseases
Olasunkanmi S. Olawuwo, Ibukun M. Famuyide, Lyndy J. McGaw
Frontiers in Veterinary Science. 2022; 9
[Pubmed] | [DOI]
7 Ayurvedic management of Dadru (dermatophytosis): A case report
Smrutimayee Sahoo, Pramod Yadav, PradeepKumar Prajapati
Journal of Ayurveda Case Reports. 2022; 5(2): 62
[Pubmed] | [DOI]
8 Evaluation for substitution of stem bark with small branches of Cassia fistula Linn for traditional medicinal uses: A comparative chemical profiling studies by HPLC, LC-MS, GC-MS
Ajay Kumar Meena, R. Ilavarasan, Ayyam Perumal, Ravindra Singh, Vikas Ojha, N. Srikanth, K.S. Dhiman
Heliyon. 2022; 8(8): e10251
[Pubmed] | [DOI]
9 Structural, Optical, Antimicrobial and Ferromagnetic Properties of Zn1-xLaxO Nanorods Synthesized by Chemical Route
S.K. Satpathy,U.K. Panigrahi,S.K. Panda,R. Biswal,W. Luyten,P. Mallick
Journal of Alloys and Compounds. 2021; : 158937
[Pubmed] | [DOI]
10 Influence of Gd doping on morphological, toxicity and magnetic properties of ZnO nanorods
S.K. Satpathy,U.K. Panigrahi,S.K. Panda,V. Thiruvengadam,R. Biswal,W. Luyten,P. Mallick
Materials Today Communications. 2021; 28: 102725
[Pubmed] | [DOI]
11 Assessment of the Antileishmanial Potential of Cassia fistula Leaf Extract
Shams Tabrez,Fazlur Rahman,Rahat Ali,Abdulaziz S. Alouffi,Bader Mohammed Alshehri,Fahdah Ayed Alshammari,Mohammed A. Alaidarous,Saeed Banawas,Abdul Aziz Bin Dukhyil,Abdur Rub
ACS Omega. 2021;
[Pubmed] | [DOI]
12 Effectiveness of medicinal plant extracts against Vibrio spp. in shrimp aquaculture
Alokesh Kumar Ghosh,Sujogya Kumar Panda,Walter Luyten
Aquaculture Research. 2021;
[Pubmed] | [DOI]
13 CCl Induced Hepatotoxicity and It's Recovery by Leaves and Seed Extract of 4Cassia Fistula
Anil Kumar R.K. Singh
Universities' Journal of Phytochemistry and Ayurvedic Heights. 2020; 2(29): 50
[Pubmed] | [DOI]
14 Antimicrobial Activity of Selected Banana Cultivars Against Important Human Pathogens, Including Candida Biofilm
Ramin Saleh Jouneghani,Ana Hortência Fonsêca Castro,Sujogya Kumar Panda,Rony Swennen,Walter Luyten
Foods. 2020; 9(4): 435
[Pubmed] | [DOI]
15 Bactericidal and Anti-biofilm Activity of Tannin Fractions Derived from Azadirachta against Streptococcus mutans
Malla Sudhakar,B. Venkata Ra
Asian Journal of Applied Sciences. 2020; 13(3): 132
[Pubmed] | [DOI]
16 Progress and prospects in the management of bacterial infections and developments in Phytotherapeutic modalities
Muhammad Akram,Muhammad Riaz,Naveed Munir,Akhtar Rasul,Muhammad Daniyal,Syed Muhammad Ali Shah,Mohammad Ali Shariati,Ghazala Shaheen,Naheed Akhtar,Farzana Parveen,Naheed Akhter,Aymen Owais Ghauri,Abdul Wadood Chishti,Muhammad Usman Sarwar,Fahad Said Khan
Clinical and Experimental Pharmacology and Physiology. 2020;
[Pubmed] | [DOI]
17 Indian medicinal plant extracts to control multidrug-resistant S. aureus, including in biofilms
Sujogya Kumar Panda,Raju Das,Rob Lavigne,Walter Luyten
South African Journal of Botany. 2020; 128: 283
[Pubmed] | [DOI]
18 Antimicrobial, radical scavenging, and dye degradation potential of nontoxic biogenic silver nanoparticles using Cassia fistula pods
Indu Singh,Seema Gupta,Hemant K. Gautam,Gagan Dhawan,Pradeep Kumar
Chemical Papers. 2020;
[Pubmed] | [DOI]
Elisa Da Silva,Leandro Nicolodi Francescato
Infarma - Ciências Farmacêuticas. 2019; 31(3): 187
[Pubmed] | [DOI]
20 Phytotoxic activity and chemical composition of Cassia absus seeds and aerial parts
I. Zribi,H. Sbai,N. Ghezal,G. Richard,D. Trisman,M. L. Fauconnier,R. Haouala
Natural Product Research. 2017; : 1
[Pubmed] | [DOI]
21 Bioactive Amento flavone isolated from Cassia fistula L. leaves exhibits therapeutic efficacy
M. Srividhya,H. Hridya,V. Shanthi,K. Ramanathan
3 Biotech. 2017; 7(1)
[Pubmed] | [DOI]
22 Effect of female group size on harem male roosting behavior of the Indian short-nosed fruit bat Cynopterus sphinx
Valliyappan Mahandran,Chinnaperamanoor Madhappan Murugan,Parthasarathy Thiruchenthil Nathan
acta ethologica. 2017;
[Pubmed] | [DOI]
23 Potent bactericidal activity of silver nanoparticles synthesized from  Cassia fistula fruit
Muhammad Imtiaz Rashid,Liyakat Hamid Mujawar,Mohammad Ibrahim Mujallid,Muhammad Shahid,Zulfiqar Ahmad Rehan,Muhammad Kashif Iqbal Khan,Iqbal M.I. Ismail
Microbial Pathogenesis. 2017; 107: 354
[Pubmed] | [DOI]
24 Epicatechin downregulates adipose tissue CCL19 expression and thereby ameliorates diet-induced obesity and insulin resistance
T. Sano,S. Nagayasu,S. Suzuki,M. Iwashita,A. Yamashita,T. Shinjo,T. Sanui,A. Kushiyama,T. Kanematsu,T. Asano,F. Nishimura
Nutrition, Metabolism and Cardiovascular Diseases. 2016;
[Pubmed] | [DOI]
25 Biogenic synthesis of silver nanoparticles from Cassia fistula (Linn.): In vitro assessment of their antioxidant, antimicrobial and cytotoxic activities
Dambarudhar Mohanta,Debashis De,Kunal Biswas,Jaya Bandyopadhyay,Akshaya Kumar Bastia,Sujogya Kumar Panda,Abiral Tamang,Yugal Kishore Mohanta
IET Nanobiotechnology. 2016;
[Pubmed] | [DOI]
26 Antibacterial efficacy of five medicinal plants against multidrug-resistant enteropathogenic bacteria infecting under-5 hospitalized children
Shakti Rath,Rabindra N. Padhy
Journal of Integrative Medicine. 2015; 13(1): 45
[Pubmed] | [DOI]
27 Antibacterial activity of Eleutherine bulbosa against multidrug-resistant bacteria
Laxmipriya Padhi,Sujogya Kumar Panda
Journal of Acute Medicine. 2015; 5(3): 53
[Pubmed] | [DOI]
28 The Antibacterial Activity of Cassia fistula Organic Extracts
Seyyed Mansour Seyyednejad,Hossein Motamedi,Mouzhan Vafei,Ameneh Bakhtiari
Jundishapur Journal of Microbiology. 2014; 7(1)
[Pubmed] | [DOI]
29 Comparative in vitro Antimicrobial and Phytochemical Evaluation of Methanolic Extract of Root, Stem and Leaf of Jatropha curcas Linn
Amit Kumar Sharma, Mayank Gangwar, Ragini Tilak, Gopal Nath, Akhoury Sudhir Kumar Sinha, Yamini Bhusan Tripathi, Dharmendra Kumar
Pharmacognosy Journal. 2012;
30 Study of plants used against the skin diseases with special reference to Cassia fistula L. among the king (Dongaria Kandha) of Niyamgiri: A primitive tribe of Odisha, India
Sanjeet Kumar, P.K.Jena, S. Sabnam, M.Kumari,P.K.Tripathy
International Journal of Drug Development & Research. 2012; 4(2): 256-264
31 Evaluation of antimicrobial activity and bronchodialator effect of a polyherbal drug-Shrishadi
Divya Kumari Kajaria, Mayank Gangwar, Dharmendra Kumar, Amit Kumar Sharma, Ragini Tilak, Gopal Nath, Yamini Bhusan Tripathi, JS Tripathi, SK Tiwari
Asian Pacific Journal of Tropical Biomedicine. 2012; : 905-909
32 Investigation of the effects of extractionsolvent/technique on the antioxidant activity of Cassia fistula L
Barkat Ali Khan, Naveed Akhtar, Akhtar Rasul, Tariq Mahmood, Haji Muhammad Shoaib Khan, Shahiq-Uz-Zaman, Muhammad Iqbal,Ghulam Murtaza
Journal of Medicinal Plants Research. 2012; 6(3): 500-503
33 Evaluation of antimicrobial activity and bronchodialator effect of a polyherbal drug–Shrishadi
Divya Kumari Kajaria,Mayank Gangwar,Dharmendra Kumar,Amit Kumar Sharma,Ragini Tilak,Gopal Nath,Yamini Bhusan Tripathi,JS Tripathi,SK Tiwari
Asian Pacific Journal of Tropical Biomedicine. 2012; 2(11): 905
[Pubmed] | [DOI]
34 In vitro antibacterial activity of various tissue types of Dumortiera hirsuta (Sw) Nees from different altitudes of eastern Himalaya
Souryadeep Mukherjee,Arijit De,Pinky Ghosh,Abhijit Dey
Asian Pacific Journal of Tropical Disease. 2012; 2: S285
[Pubmed] | [DOI]
35 Comparative in vitro Antimicrobial and Phytochemical Evaluation of Methanolic Extract of Root, Stem and Leaf of Jatropha curcas Linn
Amit Kumar Sharma,Mayank Gangwar,Ragini Tilak,Gopal Nath,Akhoury Sudhir Kumar Sinha,Yamini Bhusan Tripathi,Dharmendra Kumar
Pharmacognosy Journal. 2012; 4(30): 34
[Pubmed] | [DOI]
36 Evaluation of antimicrobial properties of four plant extracts against human pathogens
Anjali Rawani,Sudin Pal,Goutam Chandra
Asian Pacific Journal of Tropical Biomedicine. 2011; 1(1): S71
[Pubmed] | [DOI]
37 Antidiarrheal activity ofTerminalia arjunaRoxb. from India
Sujogya Kumar Panda,Sushil Kumar Dutta,Akshya Kumar Bastia
Journal of Biologically Active Products from Nature. 2011; 1(4): 236
[Pubmed] | [DOI]
38 Evaluation of antimicrobial properties of four plant extracts against human pathogens
Anjali Rawani, Sudin Pal, GoutamChandra
Asian Pacific Journal of Tropical Biomedicine. 2011; : S71-S75
[VIEW] | [PDF]
39 Synthesis, Characterization and In-Vitro Antimicrobial Evaluation of Some Novel Isoxazoline Derivatives
Rajeev Bhimwal, Anil K Sharma, Ankit Jain
JAPER. 2011; 1(5): 251-258
[VIEW] | [PDF]
40 Antimicrobial Activity of Some Novel Pyrazoline Derivatives
Biresh K Sarkar, Ritesh Patel , Upendra Bhadoriya
JAPER. 2011; 1(5): 243-250
[VIEW] | [PDF]


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  In this article
    Materials and Me...
    Results and Disc...
    Article Figures
    Article Tables

 Article Access Statistics
    PDF Downloaded1197    
    Comments [Add]    
    Cited by others 40    

Recommend this journal