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ORIGINAL ARTICLE
Year : 2010  |  Volume : 1  |  Issue : 3  |  Page : 354-357

Determination of pregabalin in human plasma by electrospray ionisation tandem mass spectroscopy


1 Department of Chemistry, V. N. S. G. University, Udhna Magdalla Road, Surat - 395 007, Gujarat, India
2 Jiwaji University, Gwalior - 474 001, M.P., India

Correspondence Address:
Gaurang R Shah
Department of Chemistry, V. N. S. G. University, Udhna Magdalla Road, Surat - 395 007, Gujarat
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0110-5558.72423

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A rapid, precise, specific, and accurate Electrospray Ionisation Tandem Mass Spectrometry (ESI-MS / MS) method has been developed and subsequently validated, for the determination of pregabalin (PB) in human plasma. Gabapentin (GB) was used as the internal standard. PB and GB were extracted from the plasma using a combination of deproteinization, using 0.1% formic acid and liquid-liquid extraction, using methylene chloride. PB and GB were separated using the Hypurity advance column (50 mm × 4.6 mm, 5 μm) and mobile phase, consisting of methanol : 0.1% formic acid (80:20 v / v). PB was determined by using ESI-MS / MS in positive ion mode, with the help of the API 2000 spectrophotometer, operated in a multiple reaction monitoring mode. The parent-to-product ion combination of m / z 160.2→55.1 and 172.2→95.0 was used to quantify PB and GB, respectively. The assay was validated in the concentration range of 99.79 - 4019.90 ng / mL for PB. The limit of quantification (LOQ) was identifiable and reproducible at 99.79 ng / mL. The method has been successfully applied to study the pharmacokinetics of PB in healthy male volunteers.


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