Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 11  |  Issue : 3  |  Page : 101--106

Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA


Gul-e-Saba Chaudhry1, Abdah Akim2, Muhammad Naveed Zafar3, MA Abdullah4, Yeong Yik Sung4, Tengku Sifzizul Tengku Muhammad4 
1 Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Terengganu; Department of Chemical Engineering, Universiti Teknologi Petronas, Perak, Malaysia
2 Department of Biomedical Sciences, Universiti Putra Malaysia, Seri Kembangan, Selangor, Malaysia
3 Department of Chemistry, Quaid-i-Azam University, Islamabad, Pakistan
4 Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Kuala Terengganu, Malaysia

Correspondence Address:
Prof. Abdah Akim
Department of Biomedical Sciences, Universiti Putra Malaysia, Seri Kembangan, Selangor
Malaysia
Dr. Gul-e-Saba Chaudhry
Institute of Marine Biotechnology, Universiti Malaysia Terengganu, Terengganu 21030
Malaysia

Cancer is a complex multifactorial disease and leading causes of death worldwide. Despite the development of many anticancer drugs, there is a reduced survival rate due to severe side effects. The nontargeted approach of convention drugs is one of the leading players in context to toxicity. Hyaluronan is a versatile bio-polymer and ligand of the receptor (CD44) on cancer cells. The MCF-7 and HT-29 cancer cell lines treated with hyaluronic acid-paclitaxel (HA-PTX) showed the distinguishing morphological features of apoptosis. Flow cytometric analysis showed that HA-PTX induces apoptosis as a significant mode of cell death. The activation level of tumor suppressor protein (p53) increased after PTX treatment in MCF-7, but no changes observed in HT-29 might be due to hereditary mutations. The lack of suppression in AKT and Rho A protein suggest the use of possible inhibitors in future studies which might could play a role in increasing the sensitivity of drug towards mutated cells line and reducing the possibilities for cancer cell survival, migration, and metastasis.


How to cite this article:
Chaudhry Ge, Akim A, Zafar MN, Abdullah M A, Sung YY, Muhammad TS. Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA.J Adv Pharm Technol Res 2020;11:101-106


How to cite this URL:
Chaudhry Ge, Akim A, Zafar MN, Abdullah M A, Sung YY, Muhammad TS. Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA. J Adv Pharm Technol Res [serial online] 2020 [cited 2020 Aug 10 ];11:101-106
Available from: http://www.japtr.org/article.asp?issn=2231-4040;year=2020;volume=11;issue=3;spage=101;epage=106;aulast=Chaudhry;type=0