Journal of Advanced Pharmaceutical Technology & Research

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 11  |  Issue : 1  |  Page : 6--12

The purity identification and radiolabeling of α-mangostin with technetium-99m


Muchtaridi Muchtaridi, Luthfi Utami Setyawati, Risda Rahmi Islamiaty, Kevin Reinard Lie, Wiwit Nurhidayah 
 Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, Indonesia

Correspondence Address:
Prof. Muchtaridi Muchtaridi
Jl. Bandung-Sumedang KM 21, Jatinangor, 45363
Indonesia

Alpha-mangostin (AM) is a natural compound that has the greatest activity in breast cancer. Radiolabeling AM with technetium-99 m (Tc-99m) has a function as breast cancer radiotracer. This study is aimed to identify the purity of Tc-99m-labeled AM. The identification method was conducted by a validated radio-high-performance liquid chromatography (HPLC) to confirm the chemical purity of the compound when the thin layer of chromatography and paper chromatography were used to find out the radiochemical purity (RCP). The validated radio-HPLC method obtained was C18 column with methanol:water (90:10) as the mobile phase and ultraviolet (243 nm) tandem radioactive detector (Gabi Star). The result showed that the RCP was 70.6% ± 2.87%. The analytical method met the validation criteria according to ICH Q2 (R1); thus, it could be applied in the identification. Unfortunately, the99mTc-AM identification using radio-HPLC showed that the expected complex was not yet formed perfectly because of chemical impurities.


How to cite this article:
Muchtaridi M, Setyawati LU, Islamiaty RR, Lie KR, Nurhidayah W. The purity identification and radiolabeling of α-mangostin with technetium-99m.J Adv Pharm Technol Res 2020;11:6-12


How to cite this URL:
Muchtaridi M, Setyawati LU, Islamiaty RR, Lie KR, Nurhidayah W. The purity identification and radiolabeling of α-mangostin with technetium-99m. J Adv Pharm Technol Res [serial online] 2020 [cited 2020 Apr 10 ];11:6-12
Available from: http://www.japtr.org/article.asp?issn=2231-4040;year=2020;volume=11;issue=1;spage=6;epage=12;aulast=Muchtaridi;type=0