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ORIGINAL ARTICLE
Year : 2020  |  Volume : 11  |  Issue : 3  |  Page : 117-122

Evaluation and quantification of the inhibition of biofilm and planktonic forms of Klebsiella pneumoniae by the polyphenolic extract of Pulicaria crispa


1 Department of Biology, Laboratory of Valorisation and Conservation of Biological Resources, University of M'hamed Bougara, Boumerdes, Algeria
2 Department of Biochemistry and Microbiology, Laboratory of Analytical Biochemistry and Biotechnology, Mouloud Mammeri University, TiziOuzou, Algeria
3 Entomology-Oncology-Genetics-IHU Méditerranée, Rickettsia Unit, URMITE UMR, Marseille, France

Correspondence Address:
Dr. Afif Chaouche Thinina
160 City, Bulding 12, N 8, Thenia, Boumerdes, 35000
Algeria
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/japtr.JAPTR_165_19

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Analyses of polyphenolic plant extracts have shown significant results when used to control different pathogens. Many of these pathogens are responsible for different infections causing significant public health problems. This work aims basically to determine the efficiency of polyphenolic extract of Pulicaria crispa to prevent biofilm formation by Klebsiella pneumoniae. Strains were identified by their biochemical characters and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. P. crispa is a Saharan plant used to extract polyphenols to assess their inhibitory action against K. pneumoniae development and biofilm forming. High-performance liquid chromatography revealed quercetin as the most important component of the polyphenolic extract. All strains are biofilm forming and are resistant to many antibiotics. The Minimal inhibitory concentrations of biofilm (MICBs) of the extract range from 0.21 mg gallic acid equivalent (GAE) to 3.40 mg GAE. The minimal inhibitory concentrations vary from 0.1 mg GAE to 0.425 mg GAE. Although many plant extracts have already shown their antimicrobial and antibiofilm activities, their application in clinical cases requires a long-term endeavor.


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