|Year : 2020 | Volume
| Issue : 1 | Page : 36-43
Polymeric worm-like nanomicellar system for accelerated wound healing
Aarti Singh1, Adeeba Shakeel2, Dakshi Kochhar3, Sampathkumar Jeevanandham4, Satyendra Kumar Rajput5, Monalisa Mukherjee3
1 Amity Institute of Click Chemistry Research and Studies, Amity University, Noida, Uttar Pradesh, India
2 All India Institute of Medical Sciences, New Delhi, India
3 Amity Institute of Click Chemistry Research and Studies; Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India
4 Amity Institute of Click Chemistry Research and Studies; Amity Institute of Nanotechnology, Amity University, Noida, Uttar Pradesh, India
5 Amity Institute of Pharmacy, Amity University, Noida, Uttar Pradesh, India
|Date of Web Publication||7-Feb-2020|
Dr. Monalisa Mukherjee
Amity Institute of Click Chemistry Research and Studies, J3 Block, Lab 103, Amity University, Noida, Uttar Pradesh
Source of Support: None, Conflict of Interest: None
Self-assembly is an unparalleled step in designing macromolecular analogs of nature's simple amphiphiles. Tailoring hydrogel systems – a material with ample potential for wound healing applications – to simultaneously alleviate infection and prompt wound closure is vastly appealing. The poly (DEAEMA-co-AAc) (PDEA) is examined with a cutaneous excisional wound model alterations in wound size, and histological assessments revealed a higher wound healing rate, including dermis proliferation, re-epithelialization, reduced scar formation, and anti-inflammatory properties. Moreover, a mechanism for the formation of spherical and worm-like micelles (WLMs) is delineated using a suite of characterizations. The excellent porosity and ability to absorb exudates impart the PDEA with reliable wound healing. Altogether, this system demonstrates exceptional promise as an infection-mitigating, cell-stimulating, homeostasis-maintaining dressing for accelerated wound healing. The aim and objective of this study is to understand the mechanism of self-assembly in synthesized WLMs from PDEA and their application in wound healing.
Keywords: Block copolymers, hydrogel, self-assembly
|How to cite this article:|
Singh A, Shakeel A, Kochhar D, Jeevanandham S, Rajput SK, Mukherjee M. Polymeric worm-like nanomicellar system for accelerated wound healing. J Adv Pharm Technol Res 2020;11:36-43
|How to cite this URL:|
Singh A, Shakeel A, Kochhar D, Jeevanandham S, Rajput SK, Mukherjee M. Polymeric worm-like nanomicellar system for accelerated wound healing. J Adv Pharm Technol Res [serial online] 2020 [cited 2020 Apr 10];11:36-43. Available from: http://www.japtr.org/text.asp?2020/11/1/36/277923
| Introduction|| |
Supramolecular assembly represents a dynamic tool for the fabrication of substantial functional complex architectures.,, The primary driving force for building self-assembled objects arises from the capacity of small and discrete entities to assemble spontaneously into more organized systems., Among the variety of self-assembled polymer architectures that are routinely utilized, linear block copolymers (BCPs) unequivocally play an eminent multifaceted role in nanoscience. Self-assembly of amphiphilic BCPs in aqueous solution is an inexpensive bottom-up strategy to create new polymeric nanostructures with seemingly endless possibilities in biomedical applications.,
A control over the self-assembly of amphiphilic BCPs typically relies on the volume fraction or ratio of hydrophobic and hydrophilic blocks along with their chain topology and architecture. The unfavorable contact between water and the hydrophobic segments results in their self-assembly into small aggregates, which exhibit a rich polymorphism, the simplest structure being spherical micelles. Micelles can emerge into flexible and elongated aggregates, referred to as worm-like micelles (WLMs). As a result of their transient nature, WLMs display unprecedented dynamic and static properties which are exploited in numerous fields.
The course of cutaneous wound healing is immensely complex, controlled by an intricate interplay between factors operating in concert to reinstate injured skin. This process occurs with the intricate reorganization of extracellular matrix networks. Owing to this convoluted repair system, normal wound healing can only support a maladaptive regeneration of human skin., Among biomaterials utilized as wound dressings, polymer-based sponges are ideal candidates for wound dressings. Herein, with a suite of characterizations, we delineate the mechanism behind the formation of spherical and WLMs from poly(DEAEMA-co-AAc) (PDEA) self-assembly which demonstrates excellent accelerated cutaneous wound healing owing to their exceptional biocompatibility and capacity to maintain homeostasis in an otherwise maladaptive healing system.
| Subjects and Methods|| |
AAc (99% pure) was purchased from Qualigen fine chemicals, India. DEAEMA (98% pure) was acquired from Aldrich, USA. Synthesis and assays were carried out in triple deionized water.
The synthesis was done through free-radical aqueous copolymerization using DEAEMA (20 mol%), AAc (80 mol%), Ammonium persulphate (APS), and Tetramethylethylenediamine (TEMED) as reported earlier. The monomer mixture was then poured into molds and kept in a water bath at 40°C for 20 h. The hydrogels were kept at −20°C overnight and lyophilized at −70°C for 48 h.
The surface morphology of the synthesized PDEA sponges was studied through scanning electron microscopy (Zeiss EVO 50). Transmission electron microscopy (TEM) micrographs were taken on a JEOL, JEM-2100F electron microscope at an acceleration voltage of 200 kV. Fourier-transform infrared (FTIR) was performed on attenuated total reflection-FTIR (ATR-FTIR) model, Nicolet-5DX F fitted with a temperature control. The samples were characterized in the wavenumber range of 4000–500 cm−1 at a fixed resolution of 4 cm−1. X-ray photoelectron spectroscopy (XPS) absorption measurements were done at BL-14 beamline of Indus 2 synchrotron radiation source, Indore. The rheology was studied by utilizing small-amplitude oscillatory shear experiments using a fixed stress of 1.0 Pa, on an AR 500 Rheometer (TA Instruments, Surrey, England). Atomic force microscope (AFM) image was obtained through a Nanoscope IIIa at IUAC, New Delhi with tapping mode.,
Lyophilized PDEA sponges were weighed (Wo) and kept in phosphate-buffered saline (PBS) solution of pH (7.4) at room temperature. The samples were removed at fixed intervals of time, and the swollen weight (Wt) was recorded till equilibrium was attained.
The equilibrium ratio at a particular time t was calculated as:
W0 = Initial dry sample weight
Wt = Final swollen sample weight
In vitro release study
PDEA sponges were immersed in benzalkonium chloride (BZC) solution prepared in PBS (1 mg/ml) till equilibrium swelling was attained. The loaded sponges were then dried and immersed in fresh PBS, and the system was kept in a shaker incubator at 75 rpm. At a fixed time interval, 2 ml of the aliquot was withdrawn from solution. The concentration of BZC released from the hydrogel was determined at 262 nm by an ultraviolet–visible spectrophotometer in triplicates.,
Cell viability assay
The prepared sponge extract obtained after incubation at 37°C for 48 h (200 μl per well in triplicates) was added to tissue culture plates (Corning). Human dermal fibroblast α (HDFα) cells were trypsinized, and the cell density was determined using a hemocytometer, and 200 μl/well of cell suspension (~3000 cells) were added to the sponge extract. After 48 h of incubation, cytotoxicity was assessed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (ThermoFisher). The readings were taken using enzyme-linked immunosorbent assay (ELISA) plate reader at 570 nm after 4 h incubation.,
T-test was used to perform statistical analysis. P < 0.05 was considered statistically significant.
Experimental protocol for evaluation of wound healing
Animals for the present investigation were approved by the Institutional Animal Ethical Committee, Amity University, Noida as per the guidelines of CPCSEA, New Delhi, India.
Wistar albino rats of either sex (weight 180–200 g) were allocated into two groups (normal control and treatment group having 6 rats/group). The control group was subjected to normal healing, whereas the treatment group received topical application of PDEA sponges (0.5 mg) swollen in normal saline (1 ml) over a circular area of 10 mm diameter was applied daily.
Creation of wound
The dorsal area of all animals was cleaned by trimming their hair and rinsed with 10% povidone-iodine solution, followed by 70% isopropyl alcohol. Animals were anesthetized by intraperitoneal injection of xylazine (10 mg/kg) and ketamine hydrochloride (25 mg/kg). A full-thickness excisional wound was created by biopsy punch (diameter = 10 mm; AccuPunch, Acuderm, USA).
Measurement of wound contraction and epithelization period
In the excision wound model, the wound area was measured by tracing the wound with the help of a transparent sheet using millimeter-based graph paper at different time points including 0, 4th, and 10th day for both groups. Wound contraction was calculated:
Assessment of immunological parameters
The estimation of various immunological biomarkers was carried by measuring interleukin-6 (IL-6), IL-10, and tumor necrosis factor-alpha (TNF-α) for the estimation of inflammatory cytokines using ELISA kits (Raybiotec, USA). Blood sample was collected from each animal, and serum was separated by centrifugation at 3,000 RPM for 10 min. All the biochemical estimations were done on fresh tissue samples.
Histological assessment was done at an alternative time point (4th day and 10th day). Wounds were removed under euthanasia by intraperitoneal injection of pentobarbital (200 mg). Animals from each group were sacrificed. Wound skins were taken for routine processing and H and E staining (fixating, dehydrating, embedding, and cutting). Slides were analyzed under light microscope (COSLAB, INDIA).
| Results and Discussion|| |
Cross-sectional morphology of the as-synthesised PDEAsponges indicates that the interior of the gels is uniformly macroporous at pH 7.4. PDEA exhibited a large average pore size primarily owing to the ionization of AAc units [Figure 1]a [Figure 1]b [Figure 1]c. The PAAc chains undergo dissociation facilitated by the ionization of the pendant groups which give rise to an electrostatic repulsion causing the network to swell. The amount of water entrapped within the polymer matrix directly corresponds to the pore size retrieved upon swelling. The greater the amount of water entrapped between the polymer networks, the larger will be the pore size obtained upon lyophilization of the swollen gel. Thus, increasing AAc content increases the average pore size of the PDEA at pH 7.4 producing sponge-like PDEA with enhanced wound exudate absorption and moisture vapor transmission.
|Figure 1: Scanning electron microscopy a photomicrographs of the poly (DEAEMA-co-AAc) (a-c) Displays uniformly distributed pores|
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The self-assembling nature of amphiphilic polymers facilitates the formation of a range of micellar morphologies attributed to two competing factors: interfacial energy between the two blocks and chain stretching. Conspicuous from the TEM images [Figure 2]a, micelles of varying sizes were observed due to the emergence of aggregates by the amphiphilic polymeric structures. As a consequence of the asymmetric diblock, an energetically favorable micellar morphology is obtained wherein poly(DEAEMA-co-AAc) hydrophobic blocks prefer to aggregate into spherical microdomains leaving pAAc to surround them as “coronas” [Figure 2]b and [Figure 2]c These individual monodisperse spherical micelles [Figure 2]b and [Figure 2]c can be considered as the starting morphology for various aggregates, insisting the nonergodic nature of copolymer amphiphiles. On further investigation of the crystalline domains of [Figure 2]c, we observed a d-spacing of 0.159 [Figure 2]d, whereas the Selected area electron diffraction (SAED) patterns reveal planes of (205), (201), (301), and (111) [Figure 2]e.
|Figure 2: (a-c) Transmission electron microscopy micrographs displaying centrosymmetric core-shell polymeric micelles. (d) Crystalline domains of Part c. (e) SAED pattern corresponding to Part d. (f) dynamic networks of worm like micelles|
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Intramolecular self-collapsing varies with polymeric chain flexibility bolstering interfacial collision of these amphiphilic blocks. The imbalance in the volume fraction of PDEA copolymer assembly is majorly due to thermodynamic instability with predominant hydrophilic domains at the corona which are superseded by the hydrophobic interactions between the cores ultimately leading to the morphological transition of micelles minimizing the entropy. Subsequently, the increase in the degree of quaternization of highly cross-linked DEAEMA blocks triggers self-collapse of micellar morphology eventually resulting in the profusion of WLMs. These elongated WLMs are energetically favorable relative to shortened cylinders with incorporated end defects, since these structures allow uniform curvature across the entire aggregate. Above a concentration threshold, the WLMs entangle into a dynamic reversible network [Figure 2]f that is constantly breaking and reforming.
The surface morphology of PDEA micelles was investigated using AFM. Few isolated domains from the topologically flat surface resembled the micellar architectures of PDEA copolymers with low surface energy [Figure 3]a. AFM images show the spherical structures with a maximum height profile of 13.69 nm, slightly larger than the thickness of ~6.2 nm [Figure 3]b, confirming the formation of spherical polymeric micelles. The spongy nature of PDEA micelles can be correlated with the dot-like disordered microdomains oriented parallel to the substrate. The low average surface roughness of 1.04 nm affirms the smooth and homogenous morphology of PDEA micelles [Figure 3]c.
|Figure 3: Atomic force microscope displaying surface topography of poly (DEAEMA-co-AAc) sponges. (a) Atomic force microscope height image with corresponding histogram. (b) corresponding height profile. (c) Three-dimensional representation|
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The structural aspects of PDEA BCPs are investigated using ATR-FTIR spectroscopy [Figure 4]. The broad and intense peak at 3448 cm-1 is attributed to intermolecular hydrogen bonding and the dimerization of saturated aliphatic acid (PAAc) groups in the polymer matrices. The sharp and intense peaks at 2925 cm-1 and 2853 cm-1 correspond to the strong asymmetrical and symmetrical stretching vibrations of methylene groups in the copolymer blocks, respectively. However, a prominent asymmetrical stretching band at 1639 cm-1 is attributed to dominant carboxylate anions (COO-) of PAAc domains supporting the polyelectrolyte complexation. The characteristic peak at 1165 cm-1 is ascribed to the symmetrical C-N-C (tertiary aliphatic amine) stretching of PDEAEMA pendant groups confirming their quaternization effect leading to strong electrolytic complexation of PDEA blocks.
The wide-scan XPS survey peaks encompass the existence of C 1s, N1s, and O1s components at 286.2 eV, 401.7 eV, and 532.7 eV, respectively [Figure 5]a. The high-resolution C1s spectrum [Figure 5]b of PDEA can be deconvoluted into four peaks, positioned at the binding energies of 284.6 eV (C=C), 285.8 eV (C-C/C-H), 287 eV (C-N/C-O), and 288.7 eV (O-C=O). The dominant C-C/C-H peaks correspond to the aliphatic carbon atoms of the long polymer backbone. However, the broad peaks of the O-C = O component affirm the presence of secondary carbon atoms of the ester groups of PDEAEMA. Meanwhile, the intensified C-N/C-O peaks represent the presence of few neutral moieties in PDEAEMA endeavoring strong electrolytic interactions with COO moieties of PAAc. Moreover, the sharp O1s spectrum of the BCP can be fitted into three Gaussian peaks centered at 531.3 eV (C = O), 532.7 eV (O-H), and 534.2 eV (C-O-C) [Figure 5]c. The predominance of C = O over C-O-C moieties confirms the entanglement of PAAc domains at the corona of polymeric micelles surrounding the hydrophobic core DEAEMA domains. Furthermore, the intensified O-H peaks arises as a consequence of intermolecular hydrogen bonding, and plausible dimerization of PAAc domains with water molecules. In addition, the N1s spectrum can also be deconvoluted into two distinct broad peaks representing significant XPS signals at 400.2 eV corresponding to neutral C-N moieties, whereas the other characteristic peak at 402.6 eV affirms the quaternization (C-N +) effect of the hydrophobic domains resulting in the formation of polyelectrolyte complexes [Figure 5]d.
|Figure 5: X-ray photoelectron studies of poly (DEAEMA-co-AAc) sponges. (a) Survey spectrum. (b) high-resolution C1s spectrum. (c) High-resolution O1s spectrum. (d) High-resolution N1s spectrum|
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The rheology pattern in [Figure 6] shows the typical plots of shear storage (G') and loss (G'') modulus versus the oscillatory frequency (ω) sweep curves. The results of frequency sweep experiments the relation G'(ω) > G''(ω), indicating spongy-like stable elastic behavior of PDEA gels. The calculated loss factor tan δ (G''/G') showing a substantial increase in values 0.1–0.16 (Gradual increase in temperature) indicates higher energy dissipation of PDEA sponges resulting slightly viscous nature to the material.
Swelling and release studies
When kept in PBS buffer of pH 7.4, the sponges initially showed fast swelling for the first 15 h and attained the equilibrium swelling after ~28H with an equilibrium swelling ratio of ~3200% [Figure 7]a. The –COOH groups of AAc bound to the polymeric chain becomes protonated forming –COO- ions. The electrostatic repulsions between the –COO– lead to the swelling of PDEA sponge network forming large pores.
|Figure 7: (a) Equilibrium swelling capacity of hydrogel nanomembrane around 3200%. (b) Cumulative drug release up to 88%|
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The drug-loaded hydrogel membranes followed a two-stage drug release profile as shown in [Figure 7]b. At physiological pH (7.4), the electrostatic repulsion between the carboxylate anions causes the network to swell and allows the entrapped drug to move outside the matrix through osmosis. The slow and extended swelling property leads to sustained release of drug with an exponential increase in concentration till ~ 25H releasing 88% of the drug.
Percentage of wound contraction and healing rate
In the treated group, the healing rate of the wound on the 4th day [Figure 8]b was greater than the control (50% and 30%, respectively). On the 10th day, complete wound closure was observed in the treated rats, whereas only 70% recovery was seen in the normal control [Figure 8]a. Moreover, no fester or hypertrophic scarring was observed at the wound site in the treated rats and the fur started to reappear by the 10th day.
|Figure 8: (a) In vivo assessment of wound healing on Wistar rat at day 0, 4, and 10 with normal control and treated groups (scale bar 10 mm) with graphical representation of (b) wound contraction percentage|
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The early phase (4th day) shows an increased level of polymorphonuclear leukocytes, polymorphonuclear neutrophils, or inflammatory cells near the excision line of the wound. The demarcation line showed the complete separation of exudate formation, and the continuous layer of epithelial cells was absent. However, in the control group, higher exudate accumulation was observed which reflects the deposition of a foreign substance and can be measured by increase leukocytes infiltration. The absence of exudates formation and lower level of leukocytes infiltration at later phase indicated that the upsurge of inflammatory cytokines release was almost finished. In addition, in comparison to normal control, a complete layer of epithelialization was observed in treated animals on the 10th day which corroborates the intense healing of the wound. Collagen deposition was significantly raised at the end of healing of the wound in treated animals. Less deposition of collagen indicated incomplete wound healing in normal control animals compared to the treated group [Figure 9].
Early re-epithelialization observed in the treated rats as compared to control was validated by the significant reduction in the concentration of IL-6 and TNF-α, and an increase in IL-10 [Figure 10] unveiling the anti-inflammatory potential of the PDEA sponges.
| Conclusions|| |
In summary, our findings delineate the intimate interaction between amphiphilic BCPs to achieve hierarchical structures that facilitate accelerated wound healing by decreasing oxygen tension, promoting wound dehydration, and reducing inflammation. Experimental procedures for the on-demand synthesis of hydrogels are undertaken after carefully setting the properties desired to attain spherical and worm-like nanomicellar structures having vital implications on their morphological and chemical characteristics as well as their biocompatibility, swelling and release, and wound healing properties, making them competitive candidates to be applied as potent wound dressings.
Financial support and sponsorship
Prof. Monalisa Mukherjee thanks the Department of Biotechnology BT/PR21866/NNT/28/1145/2016 for funding this project, and Amity University Uttar Pradesh Noida for providing research infrastructure.
Conflicts of interest
There are no conflicts of interest.
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