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ORIGINAL ARTICLE
Year : 2019  |  Volume : 10  |  Issue : 4  |  Page : 149-154

In vitro anticancer activity Annona squamosa extract nanoparticle on WiDr cells


1 Doctoral Student of Veterinary Science, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
2 Doctoral Student of Medical Science, Faculty of Medicine, Airlangga University, Surabaya, Indonesia
3 Department of Microbiology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
4 Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia

Correspondence Address:
Prof. Sri Agus Sudjarwo
Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya
Indonesia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/japtr.JAPTR_10_19

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This study aimed to prepare Annona squamosa leaf extract-loaded chitosan nanoparticles (nano-ASLE) against human colon cancer (WiDr) cells. Nano-ASLE was made with ionic gelation method. Four concentrations of the nano-ASLE (50, 100, 200, and 400 μg/mL) in dimethyl sulfoxide were prepared on WiDr cells to determine the IC50 value using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Then, it was divided into three groups of concentration of IC50, 2IC50, and 4IC50 and continued with analysis of caspase-3 expression and cell cycle arrest. The results of particles size were obtained 535.1 nm and showed potent cytotoxicity with IC50 292.39 μg/mL. The expression of caspase-3 increased significantly and caused cell cycle arrest at the G2/M phase and induced apoptosis on WiDr cells. Further studies are needed to obtain the loading efficiency, release of drug concentration, and in vivo study of nano-ASLE to suppress WiDr cells.


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