|Year : 2019 | Volume
| Issue : 2 | Page : 90-94
Comparison of the disinfectant effects of Nanosil D2 and Korsolex extra solutions on thermoset acrylic resin contaminated with Streptococcus mutans and Bacillus subtilis
Tahereh Ghaffari1, Fahimeh Hamedi Rad2, Tahereh Pirzadeh3, Gahanbaksh Asgari3
1 Department of Prosthodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran
2 Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
3 Department of Bacteriology and Virology, Tabriz University of Medical Sciences, Tabriz, Iran
|Date of Web Publication||12-Apr-2019|
Prof. Fahimeh Hamedi Rad
Department of Prosthodontics, Faculty of Dentistry, Babol University of Medical Sciences, Babol
Source of Support: None, Conflict of Interest: None
This study was conducted to compare the disinfectant effects of Nanosil D2 and Korsolex extra on thermoset acrylic resin contaminated with Streptococcus mutans and Bacillus subtilis. In this experimental study, 90 acrylic samples were made and sterilized. Two samples were cultured as a sterilization control in brain–heart infusion (BHI) and the rest of samples were divided into two groups. Samples of one group were placed in a bacterial suspension of S. mutans and the samples of another group were placed in a suspension containing B. subtilis. Each group was divided into two subgroups for immersion in Nanosil or Korsolex extra solutions. Seven samples were selected from each group at each of 30 min, 1 h, and 2 h and transferred to the BHI test tube, and their turbidity was evaluated after 24 h. SPSS 17 software was used to analyze the data, and the significance level of test was considered P < 0.05. At 1 h, Bacillus level of Nanosil D2 was significantly lower than that of Korsolex extra, and at all ½, 1, and 2 h, the level of Streptococcus in Nanosil D2 solution was significantly lower than that of Korsolex extra (P < 0.05). Bacillus and Streptococcus levels showed significant reduction in both solutions over time. The disinfecting power of Nanosil D2 is more than that of Korsolex extra.
Keywords: Bacillus subtilis, Korsolex extra, Nanosil D2, Streptococcus mutans
|How to cite this article:|
Ghaffari T, Rad FH, Pirzadeh T, Asgari G. Comparison of the disinfectant effects of Nanosil D2 and Korsolex extra solutions on thermoset acrylic resin contaminated with Streptococcus mutans and Bacillus subtilis. J Adv Pharm Technol Res 2019;10:90-4
|How to cite this URL:|
Ghaffari T, Rad FH, Pirzadeh T, Asgari G. Comparison of the disinfectant effects of Nanosil D2 and Korsolex extra solutions on thermoset acrylic resin contaminated with Streptococcus mutans and Bacillus subtilis. J Adv Pharm Technol Res [serial online] 2019 [cited 2019 May 19];10:90-4. Available from: http://www.japtr.org/text.asp?2019/10/2/90/256014
| Introduction|| |
Due to the contact with tools, individuals, and places, denture prostheses are at the high risk of contamination. Hence, preventing cross-contamination and disinfecting them are crucial. Physical methods are not as effective as chemical cleansing in reducing the number of denture-contaminating microorganisms., Denture immersion in an appropriate disinfectant for adequate time to disinfect or sterilize is an easy and effective way so that it has been stated that denture immersion in sodium hypochlorite for 5 min can destroy various microorganisms, including spore-forming bacteria and Candida albicans. A minimum of 15 min of immersion in sodium hypochlorite or glutaraldehyde has been proposed to destroy the AIDS virus and hepatitis. Different times have been proposed for denture disinfection depending on the type of bacterial contamination and the type of disinfectant.,,
Disinfectants available in the market are divided into low-level, high-level, and intermediate-level types depending on the power of the effect. Some these substances include phenolic, alcoholic, and chlorine compounds known as intermediate-level substances. These disinfectants are suitable for killing Mycobacterium, vegetative bacteria, and most of viruses and fungi. Glutaraldehyde, hydrogen peroxide (H2O2), formaldehyde, and peracetic acid are known as high-level substances, used to kill all microorganisms, but they do not kill a large number of bacterial spores. In addition, alcohols, povidone-iodine, cresols, and chlorhexidine 4% are considered as low-level disinfectants. These substances may often kill most of vegetative bacteria and some fungi and viruses over a period of time. Among the wide range of existing disinfectants, the selection of them with high efficiency and fewer side effects is crucial for dentists. Although the time of using these chemicals is effective in denture disinfecting, the time should be reduced to a minimum, since it has been shown that more time affects the mechanical properties and stability of denture color.
H2O2 is used mainly for disinfecting and sterilization. It also affects the bacteria, viruses, yeasts, and spores and available at concentrations of 3%–90%. Wide range of effect, rate of effect, and long-term effectiveness, prevention of microbial re-contamination, lack of harmful effects on humans and the environment, and lack of creating microbial resistance even in long-term uses have resulted in its superiority and differentiation compared to other disinfectants. The synergistic effect of silver H2O2 in Nanosil D2 destroys a wide range of microorganisms, including the most resistant forms of them such as spores and biofilms to the weakest of them such as the HIV and hepatitis. This property makes the Nanosil D2 unique. Nanosil D2, like glutaraldehyde, is considered among the high-level disinfectants and can destroy all microorganisms. Korsolex extra is aldehyde broad-spectrum disinfection solution used for heat-resistant and heat-sensitive tools. This solution has very high adaptive power with different materials. Thus, it can be used for disinfection of glass, ceramic, stainless steel, aluminum, plastic, and hard rubber (such as latex and silicon) and artificial materials such as acrylic.
Studies show that denture prostheses are contaminated with Staphylococcus aureus, C. albicans and Streptococcus alpha-hemolytic, Streptococcus alpha- and beta-hemolytic, Klebsiella, and Pseudomonas species, and Candida colonies. Bacillus is a Gram-positive, aerobic, and spore-forming bacterium spread everywhere. Bacillus subtilis has some resistance to disinfecting processes due to its spore-forming property. Streptococci include a major part of oral flora. These bacteria play major role in decay, oral infections, and infectious endocarditis. Streptococcus mutans is part of the natural microflora of the mouth, and it can be used as a contamination marker outside the oral cavity.
Given the different results of disinfectants with various concentrations and chemical compounds, as well as the probability of contamination of dentures with various types of microorganisms showing different resistance to chemical substances, and as no study was found to compare effects of disinfectants Nanosil and Korsolex extra on acrylic resin, this study was conducted to evaluate the disinfecting effect of Nanosil D2 on Acropars acryl contaminated with S. mutans and B. subtilis and compare it with Korsolex extra disinfectant, assuming that the disinfecting effect of them is the same.
| Methodology|| |
This descriptive-analytical study was conducted in the Prosthodontic Department of Tabriz University of Medical Sciences. In this study, 90 smooth acrylic samples of Acropars 100 (Marlik Co, Eshtehard, Iran) were made in accordance with the ISO 1567 standard with a diameter of 17 mm and height of 6 mm. The specimens were sterilized by autoclave (Tecno-Gaz Evo Baganza-Parma, Italy) at 120°C with pressure of 1.4 bars for 15 min. Then, two samples as negative control were transferred to brain–heart infusion (BHI, E. Merck, 64271 Darmstadt, Germany), and after 24 h of incubation at 37°C, they were evaluated in terms of turbidity to ensure the accuracy of sterilization [Figure 1].
The remaining 88 samples were divided into two subgroups under the same conditions: One group was immersed in a microbial suspension containing S. mutans and another group was immersed in a suspension containing B. subtilis, prepared by the Iranian Pasteur Institute, which had a bacterial level equivalent to the half MacFarland. After 5 min, the samples were removed from the suspension and washed with sterilized distilled water. Then, they were placed on a sterilized dry gas. To ensure that the samples were contaminated, two samples of each group as positive control were transferred to the BHI medium, and 24 h after incubation at 37°C, they were examined in terms of turbidity. Then, each group was divided again to two subgroups (each contained 21 samples) and one group was immersed in a container containing Nanosil D2, (KimiaFam Co, Tehran, Iran) and another group was immersed in a container containing Korsolex extra (Korsolex extra, BODE Chemie GmbH, Hartmann Co, Hamburg, Germany) and the container lid was closed.
At each time of 30 min, 1 h, and 2 h, seven samples were taken from each group and each sample was transferred to a test tube containing BHI, and after 24 h, colonies were counted [Figure 2].
The obtained information was recorded in the designed table and analyzed by SPSS 21 software (IBM Corp., Armonk, NY). Data were analyzed using Mann–Whitney and Kruskal–Wallis statistical tests.
| Results|| |
The autoclave control sample did not cause turbidity in the liquid medium.
Contamination control samples made the tube turbid after adjacency with microbes and washing.
Investigating the effect of two solutions on B. subtilis showed that there is no significant difference in Bacillus level ½- and 2-h time. However, at 1-h time, the Bacillus level of Nanosil D2 was significantly less than that of Korsolex extra (P < 0.05) [Table 1].
|Table 1: Mean number of colonies produced in culture media after placing the samples in disinfectant solutions|
Click here to view
Investigating the effect of two solutions on S. mutans showed that in all ½-, 1-, and 2-h times, this bacterium level in Nanosil D2 solution was significantly lower than that of Korsolex extra (P < 0.05). The results also showed that Bacillus and Streptococcus had a significant decrease in both solutions over time [Table 1].
| Discussion|| |
In this study, the effectiveness of two chemical disinfectants (Nanosil 0.1% and 1/0 Korsolex extra 0.1%) was evaluated based on the reduction in the number of microbial colonies. The results of this study showed that there is significant relationship between the use of Nanosil and Korsolex extra solutions and reduction in the number of bacterial colonies of S. mutans and B. subtilis (P < 0.05). The greatest effect of Nanosil D2 solution was seen on Bacillus colonies in 1-h time, whereas the greatest effect was seen on reduction of Streptococcus colonies at ½ h. In this study, it was found that the bactericidal effect of D2 Nanosil was higher. The important point in this study is the effect of this solution on specific strains such as B. subtilis, which can cause stable contamination in an environment over time with producing spore. Ganavadiya et al. examined the disinfecting effectiveness of three chemical disinfectants (glutaraldehyde, H2O2, and ethyl alcohol) and concluded that the maximum reduction in the microbial load was achieved with H2O2 at first, and then, with glutaraldehyde. This result agrees with our research.
Badrian et al. evaluated the effect of three different disinfectants on alginate contaminated samples and concluded that Epimax (with H2O2 base) showed the highest reduction and it was effective in complete elimination of microorganisms in 10 min. Although these results are in line with the results of our study owing to superiority of H2O2 in the complete elimination of microorganisms, they are consistent with those of our study due to not complete cleanup of microorganisms in our study. Differences in results might be due to the difference in disinfectants and their percentage, the microbes used, and the period.
In Nanosil D2, H2O2 prevents bacterial mass proliferation effective in periodontal disease due to antibacterial properties and oxygen releasing. Released oxygen destroys the protective membranes of the virus and the bacteria and enables the Nanosil to penetrate, the mechanism by which microorganisms are destroyed. Thus, Nanosil mouthwash acts better in anaerobic environment. Silver nanoparticles, compared to silver mass, create more contact surface and increase the antimicrobial effectiveness.,,, The small size of silver particles in Nanosil increases its microbial properties by >99%., The antimicrobial property of silver ions is strongly dependent on covalent bonds to the bacterial proteins, leading to the deposition of proteins and the inactivation of the bacteria. Nanosil-coated products such as wound dressing, surgical instruments, skeletal prostheses, and contraceptive devices have been constructed. Moreover, researchers have referred to the possibility of using nano-silver canal disinfectants in endodontic treatments.,,,
In a study conducted by Kangarlou et al. to compare the antibacterial effectiveness of a new canal cleaning solution containing nano-silver with that of sodium hypochlorite and chlorhexidine, it was shown that nano-silver solution has desirable antibacterial properties, and if its other properties are desirable, it could be used as a canal cleaning solution. Both of H2O2 and silver have synergic effects. The Nanosil manufacturer claims that it has no environmental damaging effects because the main components of this substance are water and oxygen, which are not toxic and contaminant. Nano-silver has killing effect not only on bacteria but also on a wide range of fungi, protozoa, and even viruses., As resin-base denture absorbs water and oral fluids, it can be thought that it can also absorb disinfection solutions which can later release into the mouth and cause allergic reactions. The high resistance of B. subtilis to the microwave has been reported, and this may be due to the spore-forming property of this bacillus. Bacterial spores are metabolically inactive and are particularly resistant to stressful conditions, such as heat and radiation. The exposure time should be longer than 2 min, in which destructive effects on the denture material will increase. Given the acryl longtime immersion in disinfectants in this study, further studies are needed to evaluate the destructive effects of these materials and to determine the minimum immersion time with maximum effectiveness.
| Conclusion|| |
In our study, the disinfecting power of Nanosil D2 solution at a concentration of 0.1% was higher than that of Korsolex extra.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Kulak Y, Arikan A, Kazazoglu E. Existence of Candida albicans
and microorganisms in denture stomatitis patients. J Oral Rehabil 1997;24:788-90.
Webb BC, Thomas CJ, Harty DW, Willcox MD. Effectiveness of two methods of denture sterilization. J Oral Rehabil 1998;25:416-23.
Dychala GR. Disinfection, Sterilization and Preservation. 5th
ed. Philadelphia: Lea and Febiger; 1991. p. 133.
Davis DR, Knapp JF. The significance of AIDS to dentists and dental practice. J Prosthet Dent 1984;52:736-8.
Bell JA, Brockmann SL, Feil P, Sackuvich DA. The effectiveness of two disinfectants on denture base acrylic resin with an organic load. J Prosthet Dent 1989;61:580-3.
Ebadian B, Poursina F, Saghaei S. Evaluation of disinfecting effect of 0.5% sodium hypochlorite and 2% glutaraldehyde on heat-cure acrylic resin. J Mashhad Dent Sch 2007;31:217-22.
Vahedi M, Bakianian Vaziri P, Abdolsamadi HR, Pahlavan A, Hajilooii M, Abdollahzadeh SH. Evaluation of antimicrobial effect of four disinfectant solutions on handpieces contaminated to Staphylococcus aureus, Pseudomonas aeruginosa
and Candida albicans
. J Dent Med Tehran Univ Med Sci 2008;21:132-9.
Kahn RC, Lancaster MV, Kate W Jr. The microbiologic cross-contamination of dental prostheses. J Prosthet Dent 1982;47:556-9.
McDonnell G, Russell AD. Antiseptics and disinfectants: Activity, action, and resistance. Clin Microbiol Rev 1999;12:147-79.
Website information Nanosil.ir. [homepage on the Internet]. Tehran. Available from: http://www.nanosil.ir
. [Last updated on 2018 May 31; Last cited on 2018 Aug 01].
Website information BODE SCIENCE CENTER. [homepage on the Internet]. BODE Chemie GmbH – A company of the HARTMANN GROUP. Available from: http://www.productcatalogue.bode-chemie.com
. [Last updated on 2018 Mar 21; Last cited on 2018 Aug 10].
Powell GL, Runnells RD, Saxon BA, Whisenant BK. The presence and identification of organisms transmitted to dental laboratories. J Prosthet Dent 1990;64:235-7.
Dar-Odeh NS, Shehabi AA. Oral candidosis in patients with removable dentures. Mycoses 2003;46:187-91.
Hogg S. Essential Microbiology. 2nd
ed. John Wiley & Sons, Chichester, West Sussex, England. 2013. p. 403-19.
da Silva FC, Kimpara ET, Mancini MN, Balducci I, Jorge AO, Koga-Ito CY, et al.
Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. J Prosthodont 2008;17:627-33.
Website information marlic.com. [homepage on the Internet]. Tehran Marlic Medical Ind. Co. Available from: http://www. Marlic.com
. [Last updated on 2018 Jul 8; Last cited on 2018 Aug 08].
Ganavadiya R, Chandra Shekar BR, Saxena V, Tomar P, Gupta R, Khandelwal G, et al.
Disinfecting efficacy of three chemical disinfectants on contaminated diagnostic instruments: A randomized trial. J Basic Clin Pharm 2014;5:98-104.
Badrian H, Ghasemi E, Khalighinejad N, Hosseini N. The effect of three different disinfection materials on alginate impression by spray method. ISRN Dent 2012;2012:695151.
Esfahanian V, Mohamadi F, Amini SH. An in vitro
comparison of antimicrobial effect of nanosil and chlorhexidine mouthrinses. J Islam Dent Assoc Iran 2012;24:188-92.
Shrivastava S, Bera T, Roy A, Singh G, Ramachandrarao P, Dash D. Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 2007;18:1-9.
Sondi I, Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: A case study on E. coli
as a model for gram-negative bacteria. J Colloid Interface Sci 2004;275:177-82.
Panacek A, Kvítek L, Prucek R, Kolar M, Vecerova R, Pizúrova N, et al.
Silver colloid nanoparticles: Synthesis, characterization, and their antibacterial activity. J Phys Chem B 2006;110:16248-53.
Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, et al.
The bactericidal effect of silver nanoparticles. Nanotechnology 2005;16:2346-53.
Shantiaee Y, Maziar F, Dianat O, Mahjour F. Comparing microleakage in root canals obturated with nanosilver coated gutta-percha to standard gutta-percha by two different methods. Iran Endod J 2011;6:140-5.
Shantiaee Y, Dianat SO, Mohammad Khani H, Akbarzadeh Baghban A. Cytotoxicity comparison of nanosilver coated gutta-percha with guttaflow and normal gutta-percha in L929 fibroblasts with MTT assay. J Dent Sch 2011;29:63-9.
Lotfi M, Vosoughhosseini S, Ranjkesh B, Khani S, Saghiri MA, Zand V. Antimicrobial efficacy of nanosilver, sodium hypochlorite and chlorhexidine gluconate against Enterococcus faecalis
. Afr J Biotechnol 2011;10:6799-803.
Padachey N, Patel V, Santerre P, Cvitkovitch D, Lawrence HP, Friedman S, et al.
Resistance of a novel root canal sealer to bacterial ingress in vitro
. J Endod 2000;26:656-9.
Kangarlou A, Tashfam B, Naseri M, Dianat O, Taheri S.In vitro
comparison of antibacterial efficacy of a new irrigation solution containing nanosilver with sodium hypochlorite and chlorhexidine. J Dent Sch 2013;31:1-7.
Kim HS, Kang HS, Chu GJ, Byun HS. Antifungal effectiveness of nanosilver colloid against rose powdery mildew in greenhouses. Solid State Phenomena 2008;135:15-8.
Lara HH, Ayala-Nuñez NV, Ixtepan-Turrent L, Rodriguez-Padilla C. Mode of antiviral action of silver nanoparticles against HIV-1. J Nanobiotechnology 2010;8:1.
Greenwald D. Reducing infection risk in colonoscopy. Gastrointest Endosc Clin N Am 2010;20:603-14.
Najdovski L, Dragas AZ, Kotnik V. The killing activity of microwaves on some non-sporogenic and sporogenic medically important bacterial strains. J Hosp Infect 1991;19:239-47.
[Figure 1], [Figure 2]