|Year : 2015 | Volume
| Issue : 3 | Page : 88-96
Application of quality by design approach to optimize process and formulation parameters of rizatriptan loaded chitosan nanoparticles
Ajinath Eknath Shirsat1, Sohan S Chitlange2
1 Department of Pharmacy, JJTU, Rajasthan, India
2 Department of Quality Assurance, Padm. Dr. D. Y. Patil Institute of Pharmaceutical Science and Research, Pimpri, Pune, Maharashtra, India
|Date of Web Publication||27-Jul-2015|
Ajinath Eknath Shirsat
Department of Pharmacy, JJT University, Jhunjhunu, Rajasthan - 333 001
Source of Support: None, Conflict of Interest: None
The purpose of present study was to optimize rizatriptan (RZT) chitosan (CS) nanoparticles using ionic gelation method by application of quality by design (QbD) approach. Based on risk assessment, effect of three variables, that is CS %, tripolyphosphate % and stirring speed were studied on critical quality attributes (CQAs); particle size and entrapment efficiency. Central composite design (CCD) was implemented for design of experimentation with 20 runs. RZT CS nanoparticles were characterized for particle size, polydispersity index, entrapment efficiency, in-vitro release study, differential scanning calorimetric, X-ray diffraction, scanning electron microscopy (SEM). Based on QbD approach, design space (DS) was optimized with a combination of selected variables with entrapment efficiency > 50% w/w and a particle size between 400 and 600 nm. Validation of model was performed with 3 representative formulations from DS for which standard error of − 0.70-3.29 was observed between experimental and predicted values. In-vitro drug release followed initial burst release 20.26 ± 2.34% in 3-4 h with sustained drug release of 98.43 ± 2.45% in 60 h. Lower magnitude of standard error for CQAs confirms the validation of selected CCD model for optimization of RZT CS nanoparticles. In-vitro drug release followed dual mechanism via, diffusion and polymer erosion. RZT CS nanoparticles were prepared successfully using QbD approach with the understanding of the high risk process and formulation parameters involved and optimized DS with a multifactorial combination of critical parameters to obtain predetermined RZT loaded CS nanoparticle specifications.
Keywords: Central composite design, chitosan, design space, ionic gelation, quality by design, rizatriptan
|How to cite this article:|
Shirsat AE, Chitlange SS. Application of quality by design approach to optimize process and formulation parameters of rizatriptan loaded chitosan nanoparticles. J Adv Pharm Technol Res 2015;6:88-96
|How to cite this URL:|
Shirsat AE, Chitlange SS. Application of quality by design approach to optimize process and formulation parameters of rizatriptan loaded chitosan nanoparticles. J Adv Pharm Technol Res [serial online] 2015 [cited 2019 Aug 23];6:88-96. Available from: http://www.japtr.org/text.asp?2015/6/3/88/157983
| Introduction|| |
Rizatriptan (RZT) is a potent and selective 5-hydroxytryptamine 1B/1D receptor agonist for the treatment of acute migraine headaches in adults and it is considered better than the traditional triptans for the treatment of an acute migraine attack.  The bioavailability of RZT is about 45% due to first-pass metabolism and half-life is of 2-3 h.  Nasal drug delivery used to achieve longer retention of drug and brain targeting through the olfactory mucosa of the nasal cavity. Different approaches implemented includes bioadhesive gel, in-situ gel,  dry powder,  microparticles,  nanoparticles , using biodegradable and/or nonbiodegradable-natural (alginate, chitosan [CS], starch, dextran)  and/or synthetic (carbopol, poloxamer, PLGA, PLA, poly-caprolactone)  polymers. Nanoparticles have better advantages as it might cross the nasal mucosal epithelium intact, overlaying the mucosal associated lymphoid tissue, which can provide sustained release for drug for long period of time better than that of microspheres and other dosage forms. ,
Chitosan is nontoxic, biodegradable, biocompatible, hydrophilic, and it has antibacterial activity, protein affinity, positive polyanions and also approved as GRAS by the USFDA. Furthermore, it has been reported that the bioavailability of drug, proteins, and vaccines was raised by opening the tight junctions of epithelial cell layers and increasing the retention time of drug delivery locally using CS as polymer. ,,, Ionic gelation method involves ionic cross-linking of amino groups of CS and phosphate groups of tripolyphosphate (TPP), to form spontaneous gelation in aqueous solution. , Many properties of CS nanoparticles such as surface morphology, entrapment, and release characteristics are highly related to formulation and process parameters, such as concentration and molecular weight of CS, pH and concentration of cross-linker agent, curing time, stirring time, and speed. , Quality by design (QbD) approach can be applied for better understanding of the process and formulation variables, which can lead to better and robust quality into the product assuring the target quality product profile. Based on risk assessment of process and formulation variables, design of experimentation (DoE) study need to conduct on critical parameters to establish certain ranges for critical parameters within certain range to obtain design space (DS). ,,,,
In present study, we aimed to develop RZT CS nanoparticles formulation using QbD approach to understand the effect of process and formulation variables on critical quality attributes (CQAs) of RZT CS nanoparticles and to establish DS with accepted Quality Target Product Profile (QTPP).
| Materials and methods|| |
Rizatriptan, CS was supplied as gift sample from Cipla Ltd. (Mumbai, India) and Central Institute of Fisheries Technology, Cochin, India (medium molecular weight, 95% deacetylated) respectively. All other excipients, solvents were of pharmaceutical and analytical grade.
Formulation of rizatriptan chitosan nanoparticles
Nanoparticles were prepared using modified ionic gelation method, , where CS was dissolved in 1% acetic acid solution to a various concentration and TPP was dissolved in distilled water with various concentrations, based on the results of preliminary study. RZT was uniformly dispersed in TPP solution and this solution was added drop-wise to CS solution under continuous stirring at room temperature. RZT CS nanoparticles formed based on the principle of electrostatic attraction between positively charged primary amino groups on CS chains and charged polyanions (TPP). RZT CS nanoparticles were centrifuged at 6000 rpm for 30 min (Remi R-88). The supernatant liquid was separated and nanoparticles were redispersed in PBS at pH 6.8 and ultrasonicated for 5 min to disaggregate the CS nanoparticles. Three nanoparticles optimized batches, were redispersed in deionized water containing 1% w/v mannitol as cryoprotectant, and lyophilized primarily for 12 h at −20°C and secondary for 36 h at − 54°C with vacuum pressure of 0.001 mbar using Christ freeze-dryer (Christ Alpha 1-2 LD). Nanoparticles were collected, kept in glass vials and stored in dessicator.
Optimization of rizatriptan chitosan nanoparticles
The QTPP is an essential element of a QbD approach and forms the basis of design of the product. QTPP for RZT CS nanoparticles were presented in [Table 1] considering the formulation and process to develop nanoparticles.
Initial risk assessment of process parameters and formulation components of RZT CS nanoparticles was performed to identify critical parameters and components having a high-risk of impacting the drug product CQAs. High-risk parameters to the CQAs of RZT CS nanoparticles were further evaluated by performing experiments as per the DoE to reduce the risk.
Optimization using central composite design
Based on risk assessment and preliminary studies, optimization of three high-risk parameters at more than 3 levels needed to identify main and interaction effect of selected parameters on responses with minimum number of runs. Central composite design (CCD) was selected for RZT CS nanoparticles with % CS (X1), % TPP (X2), and stirring speed (X3) at 3 levels and 2 more levels as star points (−α, +α) was selected as shown in [Table 2]. The obtained RZT CS nanoparticles suspensions were further evaluated for particle size, entrapment efficiency.
Optimization of design space and validation of model
Design space was generated by setting acceptance criteria to CQAs. The 3 optimization formulations were prepared within DS and compared with predicted results of the responses and percentage error was calculated to validate the selected model.
The entrapment efficiency was determined by measuring the amount of unentrapped drug in the supernatant recovered after centrifugation (Remi R-88) of prepared RZT loaded CS nanoparticles by UV spectrophotometer at 227 nm wavelength. Results obtained were reported in triplicates.
Characterization of rizatriptan chitosan nanoparticles
Nanoparticles were analyzed for particle size, polydispersity index (PDI) using particle size analyzer (Malvern ZS 90); each time with fresh polystyrene cuvette and sample (n) = 3 as per the SOP.
Morphology of RZT CS nanoparticle was observed and photographed using SEM (JFC-1100, JEOL, University of Pune). Nanoparticles were coated with gold (<20 nm thick) using sputter for 5 min at 20 mA, an accelerating voltage of 5 kV, a working distance of 10 mm, at argon atmosphere in a high-vacuum evaporator at × 20,000.
Differential scanning calorimetric (DSC) carried out with a thermal analysis data system (DSC 2920, TA Instruments, Alzenau, Germany). The endothermic melting temperature for RZT, CS, physical mixture of RZT/CS, and RZT CS nanoparticles was determined. 10 mg of samples were scanned from 20°C to 270°C at a rate of 10°C/min and thermograms were recorded.
Powder X-ray diffraction (XRD) patterns were performed using X-ray diffractometer (a Philips 171) with a copper target and nickel filter was used to obtain XRD result for the samples. Powder were mounted on aluminum stages with glass bottoms and smoothed to a level surface. XRD pattern was measured from 10 to 500 at 2è using a step increment of 0.1 0 (2è) and a dwell time of 1 s at each step and XRD patterns were recorded.
In vitro drug release
The in-vitro drug release studies were performed using following method. , RZT CS nanoparticles were suspended in PBS at pH 7.4 and free RZT centrifuged to collect nanoparticles and resuspended in PBS. The nanoparticle was poured in dialysis tube and tied at both end (HiMedia, Mumbai) with cut-off of 12 kDa and kept in 50 ml PBS at pH 7.4 and placed in bath shaker at 37°C for 60 h. An aliquot of release medium withdrawn using syringe at different time interval including 1, 3, 6, 9, 12, 24, 36, 48, 60 h and replaced with equal amount of fresh release medium. The concentration of RZT was quantified using UV/VIS spectrophotometer at 227 nm.
Optimized formulations from DS were subjected to accelerated stability testing as per ICH guidelines at a temperature 40 ± 2°C and RH 25 ± 5% for a period of 3 Months. Nanoparticles were filled in sealed glass vials and kept in stability chamber (specifications - capacity 200 L, temperature 10-60°C, humidity 40-95%) and were analyzed for particles size, zeta potential, and entrapment efficiency as per SOP.
All obtained data were analyzed by the Student's t-test (α = 0.05) and calculated values were expressed as their mean ± standard deviation for statistical significance.
| Result and discussion|| |
Rizatriptan CS nanoparticles were prepared successfully using ionic gelation method with certain advantages as compared to other solvent based preparation methods due to the absence of nontoxic solvent, higher yield, better entrapment, and easy method. Risk assessment, an element of QbD approach was implemented to get a detailed insight of critical material attributes and critical process parameters on the CQAs based on the few preliminary experiments and knowledge space as shown in [Table 3]. Based on different criteria's such as type of study, that is, optimization, nature (process and formulation parameters), and number (3) of critical parameters and their levels (>2 levels), type of effect to know (main and interaction effect), feasibility of time and cost involvement, CCD (rotatable α ± 1.4142) was selected as compared to factorial design, Taguchi, placket Burman designs. ,,,, 20 experiments were conducted at 5 levels for each factor (−α, −1, 0, +1, +α) which provide excellent predictability, shield to missing data and giving main and interaction effects of critical parameters on response.
From preliminary screening, experimental levels of critical parameters were established between 2 and 4% of CS, 1-3% of TPP and 600-1000 rpm, stirring speed. 100% fraction of design space indicates that the design will provide a fitted response surface that is precise throughout the region of interest at 99% TI [Figure 1].
|Figure 1: Fraction of design space plot from central composite design of rizatriptan loaded chitosan nanoparticles|
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Response surface analysis for particle size and entrapment efficiency
Particle size histogram shows single peak with good intensity and narrow PDI of 0.377 [Figure 2]. Particle size was significantly affected by % TPP and stirring speed as compared to % CS, which can be depicted from "P > F" value from ANNOVA [Table 4]. All batches showed higher particle size values; with increase in % TPP and decrease in particle size with increase in stirring speed [Figure 3]a whereas model follow quadratic polynomial equation [Table 5] (Model P > F < 0.05). The effect of % TPP on nanoparticles size can be justified as, at augmented level of % TPP and CS, more electrostatic attraction between negatively charged TPP and the positively charged amino groups of CS, this could make CS chains too close together and forming multilayer around itself resulting into increase in particle size of matrix nanoparticles.  Further, the effect of stirring speed on particle size observed was prominent might be due to breaking of CS-TPP complex to smaller particle at higher stirring speed.
|Figure 2: Particle size histogram of rizatriptan chitosan nanoparticles (RCN 12)|
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|Figure 3: Three-dimensional surface plot of (a) particle size, (b and c) entrapment efficiency of rizatriptan chitosan nanoparticles|
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Entrapment efficiency of RZT loaded CS nanoparticles was mainly augmented by % TPP and % CS in the formulation (67.15% at 3% CS, 3.68% TPP at 900 rpm) (P > F < 0.05). Higher concentration of TPP leads to formation of strong poly-electrolytic matrix with positively charged amino group of CS enhancing drug entrapment efficiency as shown in three-dimensional (3D) surface plot [Figure 3]b, the same results were reported by Luo et al.  From the polynomial equation, it can be concluded that, the interactive effect of variables was relatively low as compared to main effect of variables on entrapment efficiency. Whereas stirring speed had significant effect on entrapment efficiency as it was 52.94% at low level of stirring speed and reduced to 44.28% at higher level of stirring speed but less as compared to other variables presented in 3D surface plot [Figure 3]c.
In-vitro drug release
The effect of % CS and % TPP on drug release from nanoparticles is shown in [Figure 4]. RZT release was decreased with increase in both % CS and % TPP, because of strong poly-electrostatic attraction between polymer and polyanions which leads to the formation of strong matrix. Nanoparticles exhibited almost 100% drug release in 9-12 h at low level of % CS and % TPP. Whereas at 3.68% (+α) TPP and 3% CS drug was retarded maximum up to 60 h as compared to other combination. The drug release profile exhibit the biphasic patterns, initial 20-25% burst release of drug in 3-4 h might be attributed to that of surface bound and superficial embedded drug in CS nanoparticles, and later sustained drug release up to 60 h was observed due to strong cross-linking complex of CS and TPP resulting into denser particle which retards RZT release due to swelling of CS which lowers the membrane permeability for 4RZT. The similar pattern was observed in previous studies. ,
|Figure 4: In-vitro drug release of rizatriptan chitosan nanoparticles (*RCN – RZT CS nanoparticles)|
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X-ray diffraction graph for RZT, CS, TPP and RZT loaded CS nanoparticles is shown in [Figure 5]. XRD of RZT at 2è shown crystalline nature with sharp peaks at 17°, 19°, 21°, 23°, and 25.5°. CS shows two peaks at 2è of 10°, 21° exhibit the amorphous nature, whereas TPP shows some sharp characteristic peaks at 2è of 19°, 25°, 34°, and 35° and nanoparticles formulation shows peaks at same degree as of pure drug RZT but with almost half intensity as compared to pure drug might be due to matrix formation with polymer. This indicates that there was no any interaction of drug and polymer in RZT CS nanoparticles.
|Figure 5: X-ray diffraction pattern (a) rizatriptan (RZT), (b) chitosan (CS), (c) tripolyphosphate, and (d) RZT CS nanoparticles|
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Scanning electron microscopy
Freeze - dried nanoparticles with mannitol as a cryoprotectant in powder formulation appears to be slightly spherical and rough [Figure 6] which could be due to CS as a natural polymer with less elasticity compared to synthetic polymer. SEM photographs showed CS nanoparticles adhered to mannitol particles, further optimization of freeze drying process may lead to free flowing nanoparticles.
|Figure 6: Scanning electron microscopy of rizatriptan chitosan nanoparticles|
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Differential scanning calorimetry
Differential scanning calorimetric thermogram of pure drug RZT [Figure 7] showed a sharp endothermic peak at its melting point at 178-180°C revealing the crystalline nature. CS thermogram reveals the amorphous nature with a peak around 75-80°C. Physical mixture of RZT, CS, and TPP showed less intense peak at the melting point of drug, whereas RZT CS nanoparticles exhibit peak with change in intensity.
|Figure 7: Differential scanning calorimetric thermograms for (a) rizatriptan (RZT), (b) chitosan (CS), (c) RZT + CS physical mixture, and (d) RZT CS nanoparticles|
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Optimization of design space and validation of model
Design space was generated [Figure 8] with the acceptance criteria particle size in narrow range of 350-650 nm; % entrapment efficiency >50%. Checkpoint formulations were evaluated for particle size and entrapment efficiency and compared with predicted values yielding the percentage error between −0.75 and 3.29. Thus, the low magnitudes of error in the current study indicated a high prognostic ability of CCD model in the optimization of RZT CS nanoparticles [Table 6].
Particle size were slightly increased on stability might be due to attraction of small nanoparticles together leading to increased nanoparticles. Whereas PDI obtained within 0.142-0.226 for three batches indicated good polydispersity after stability. Zeta potential data for initial period was showing range from + 32 to + 35 considered as stabile, whereas on 3M stability zeta values remains unchanged as shown in [Table 7] revealing the stable formulation. Entrapment efficiency of RZT was slightly decreased after 3 M of stability study but it was within accepted specification for RZT.
| Conclusion|| |
In this study, QbD approach was successfully implemented to gain understanding the effect of process and formulation parameters in the development of RZT CS nanoparticles. Checkpoint formulations for RZT CS nanoparticles prepared within DS showed acceptable results with the assurance of product quality. CCD RSM design provided the effect of critical parameters within and outside its levels of variables on the CQAs with certain advantages such as no data missing and obtaining two-factor interactions. Moreover, QbD approach improved the elucidation of critical parameters as compared to routine one factor at a time study. Accelerated stability data of optimized nanoparticles showed stability of RZT loaded CS nanoparticles, which lead to better drug product formulation. In further research, in-vivo animal study can be carried out on RZT CS nanoparticles to obtain bioavailability data to provide more detailed insights of CS-based nasal drug delivery.
| Acknowledgments|| |
Author would like to acknowledge and thank to Central Institute of Fisheries Technology, Cochin, and Cipla Ltd., Mumbai, India for providing CS and RZT, respectively.
| References|| |
Sanders-Bush E, Mayer SE. 5-hydroxytryptamine (Serotonin): Receptor agonists and antagonists. In: Brunton LL, Lazo JS, Parker KL, editors. Goodman and Gilman′s The Pharmacological Basis of Therapeutics. 11 th
ed. New York: McGraw Hill; 2006. p. 305-9.
Günbeyaz M, Faraji A, Ozkul A, Purali N, Senel S. Chitosan based delivery systems for mucosal immunization against bovine herpesvirus 1 (BHV-1). Eur J Pharm Sci 2010;41:531-45.
Klas SD, Petrie CR, Warwood SJ, Williams MS, Olds CL, Stenz JP, et al.
A single immunization with a dry powder anthrax vaccine protects rabbits against lethal aerosol challenge. Vaccine 2008;26:5494-502.
Kang ML, Jiang HL, Kang SG, Guo DD, Lee DY, Cho CS, et al.
Pluronic F127 enhances the effect as an adjuvant of chitosan microspheres in the intranasal delivery of Bordetella bronchiseptica
antigens containing dermonecrotoxin. Vaccine 2007;25:4602-10.
Khatri K, Goyal AK, Gupta PN, Mishra N, Vyas SP. Plasmid DNA loaded chitosan nanoparticles for nasal mucosal immunization against hepatitis B. Int J Pharm 2008;354:235-41.
Prego C, Paolicelli P, Díaz B, Vicente S, Sánchez A, González-Fernández A, et al.
Chitosan-based nanoparticles for improving immunization against hepatitis B infection. Vaccine 2010;28:2607-14.
Dudhania AR, Kosaraju SL. Bioadhesive chitosan nanoparticles: Preparation and characterization. Carbohydr Polym 2010;81:243-51.
Ding J, Na L, Mao S. Chitosan and its derivatives as the carrier for intranasal drug delivery. Asian J Pharma Sci 2012;7:349-61.
Rinaudo M. Chitin and chitosan: Properties and applications. Prog Polym Sci 2006;31:603-32.
Bansal V, Sharma PK, Sharma N, Pal OP, Malviya R. Applications of chitosan and chitosan derivatives in drug delivery. Adv Biol Res 2011;5:28-37.
Wu Y, Yang W, Wang C, Hu J, Fu S. Chitosan nanoparticles as a novel delivery system for ammonium glycyrrhizinate. Int J Pharm 2005;295:235-45.
Taranejoo S, Janmaleki M, Rafienia M, Kamali M, Mansouri M. Chitosan microparticles loaded with exotoxin a subunit antigen for intranasal vaccination against Pseudomonas aeruginosa
: An in vitro
study. Carbohydr Polym 2010;83:1854-61.
Katas H, Alpar HO. Development and characterisation of chitosan nanoparticles for siRNA delivery. J Control Release 2006;115:216-25.
Xu Y, Du Y. Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles. Int J Pharm 2003;250:215-26.
Ko JA, Park HJ, Park YS, Hwang SJ, Park JB. Chitosan microparticle preparation for controlled drug release by response surface methodology. J Microencapsul 2003;20:791-7.
Bose A, Wong TW, Singh N. Formulation development and optimization of sustained release matrix tablet of Itopride HCl by response surface methodology and its evaluation of release kinetics. Saudi Pharm J 2013;21:201-13.
Monajjemzadeh F, Hamishehkar H, Zakeri-Milani P, Farjami A, Valizadeh H. Design and optimization of sustained-release divalproex sodium tablets with response surface methodology. AAPS PharmSciTech 2013;14:245-53.
Dhillon GS, Kaur S, Sarma SJ, Brar SK. Recent development in applications of important biopolymer chitosan in biomedicine, pharmaceuticals and personal care products. Curr Tissue Eng 2013;2:20-40.
Yue S, Su-Fang T, Li-Qin G. Optimization of release of salicylic acid calibrator tablets in flow-through cell with central composite design. Chin J Pharm Anal 2009;29:1243-7.
Boonyo W, Junginger HE, Neti W, Polnok A, Pitaksuteepong T. Preparation and characterization of particles from chitosan with different molecular weights and their trimethyl chitosan derivatives for nasal immunization. J Met Mater Miner 2008;18:59-65.
Zarifpour M, Hadizadeh F, Iman M, Tafaghodi M. Preparation and characterization of trimethyl chitosan nanospheres encapsulated with tetanus toxoid for nasal immunization studies. Pharma Sci 2013;18:193-8.
Jafarinejad S, Gilani K, Moazeni E, Ghazi-Khansari M, Najafabadi AR, Mohajel N. Development of chitosan-based nanoparticles for pulmonary delivery of itraconazole as dry powder formulation. Powder Technol 2012;222:65-70.
Luo Y, Zhang B, Cheng W, Wang Q. Preparation, characterization and evaluation of selenite-loaded chitosan/TPP nanoparticles with or without zein coating. Carbohydr Polym 2010;82:942-51.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8]
[Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6], [Table 7]