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SHORT COMMUNICATION
Year : 2014  |  Volume : 5  |  Issue : 3  |  Page : 147-149

A simple reversed phase high-performance liquid chromatography (RP-HPLC) method for determination of curcumin in aqueous humor of rabbit


1 Department of Veterinary Pharmacology and Toxicology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India
2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Sciences and Animal Husbandry, Mhow, Madhya Pradesh, India
3 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Sciences and Animal Husbandry, Agartala, Tripura, India
4 Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India

Correspondence Address:
Tapan Kumar Mandal
Department of Pharmacology and Toxicology, West Bengal University of Animal and Fishery Sciences, Kolkata - 700 037, West Bengal
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-4040.137448

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This article describes a simple and rapid method for determination of curcumin (diferuloylmethane) in aqueous humor of rabbit using high-performance liquid chromatography (HPLC). Analysis was performed using a C-18 column (250 × 4.6 mm, 5 μ luna) by isocratic elution with a mobile phase containing 25 mM potassium dihydrogen orthophosphate (pH 3.5): Acetonitrile (40:60) and detection at 424 nm using a photodiode array (PDA) detector for curcumin. The regression data for curcumin showed a good linear relationship with r 2 > 0.998 over the concentration range of 0.1-10 μg ml−1 . Relative standard deviations (RSD) for the intraday and interday coefficient of variations for the assay were less than 5.0 and 8.5, respectively. The recovery of the method was between 79.8-83.6%. The quantification limit of the method for curcumin was 0.01 μg ml−1 . This method has good accuracy, precision, and quantitation limit. It is also concluded that the method is useful for measuring very low curcumin concentrations in aqueous humor.


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