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ORIGINAL ARTICLE
Year : 2013  |  Volume : 4  |  Issue : 3  |  Page : 160-165

Method development for Lawsone estimation in Trichup herbal hair powder by high-performance thin layer chromatography


1 Department of Pharmacy, Sumandeep Vidyapeeth, Pipariya, Waghodiya, Gujarat, India
2 Vasu Research Centre (A Division of Vasu Healthcare Pvt. Ltd.), 896/A, G.I.D.C, Makarpura, Vadodara, Gujarat, India

Correspondence Address:
Bhavna R Solanki
Vasu Research Centre (A Division of Vasu Healthcare Pvt. Ltd.) 896 /A, G.I.D.C., Makarpura, Vadodara - 390 010, Gujarat
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2231-4040.116780

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A simple, specific, accurate, precise and robust high-performance thin-layer chromatographic method has been developed and validated for estimation of Lawsone in Trichup herbal hair powder (coded as a THHP), polyherbal formulation. The chromatographic development was carried out on aluminum plates pre-coated with silica gel 60F 254 and good resolution was achieved with Toluene: Ethyl acetate: Glacial acetic acid (8:1:1 v/v/v) as mobile phase. Lawsone detection was carried out densitometrically at 277 nm and obtained retardation factor value was 0.46 ± 0.02. The method was validated with respect to specificity, linearity, accuracy, precision and robustness. The calibration curve was achieved to be linear over a range of 5-60 μg/ml and regression coefficient was obtained 0.998. Accuracy of chromatographic method was evaluated by standard addition method; recovery was obtained 99.25 ± 0.61% . The peak purity of Lawsone was achieved 0.999 r. Relative standard deviation for intraday and inter-day precision was 0.37-0.56% and 0.42-0.55%, respectively. The limit of detection and limit of quantification of the Lawsone were found to be 1.08 μg/m land 3.28 μg/ml, respectively. This result shows that the method was well validated. In the present study, the Lawsone content was found 0.322 ± 0.014% in THHP. This study reveals that the proposed high performance thin layer chromatography method is accurate, fast and cost- effective for routine estimation of Lawsone in polyherbal formulation.


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